OCT 01, 2015 12:00 PM PDT

Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR

Speaker
  • Professor and Director, Division of Medical Physics and Biophysics, Director, DNA technology and SARRP pre-clinical laboratories, Brigham and Women's Hospital, Dana-Farber Cancer Institute
    Biography
      Dr. Makrigiorgos is a Professor of Radiation Oncology and Director of the Medical Physics & Biophysics division at Dana Farber Cancer Institute and Brigham and Women's Hospitals, Harvard Medical School. He also directs the DNA technology laboratory and the radiation pre-clinical facility. His research interests include the development of novel DNA technologies for molecular diagnostics in Oncology and he identification of circulating cancer biomarkers. He is the inventor of several PCR-based techniques for molecular diagnostics, including Balanced-PCR, Anti-primer-quenching-real-time PCR, Hairpin-PCR, COLD-PCR and DiSSeCT technology. He is a Member of the Editorial Board of Clinical Chemistry and has published over 120 articles, reviews and book chapters.

    Abstract

    Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challenging task, especially when the position and type of mutation are unknown. We describe simple methods for enrichment of mutations in a sample, such that downstream detection or sequencing of mutations is facilitated. Using COLD-PCR, the sensitivity of almost all mutation detection methods is improved including targeted sequencing, digital PCR, and high resolution melting. Learning objectives include the adaptation of COLD-PCR to boost the mutation detection sensitivity for all these established platforms; and practical tips to get the most out of the combined technologies.


    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    JUN 17, 2020 1:30 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 17, 2020 1:30 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Understanding the complex interplay between a pathogen and the host response is important to developing effective vaccines and therapeutics. The nCounter® Analysis System and GeoMx®...
    FEB 19, 2020 11:00 AM PST
    C.E. CREDITS
    FEB 19, 2020 11:00 AM PST
    DATE: February 19, 2020TIME: 11:00am PST, 2:00pm EST...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    Loading Comments...
    Show Resources