OCT 01, 2015 12:00 PM PDT

Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR

Speaker
  • Professor and Director, Division of Medical Physics and Biophysics, Director, DNA technology and SARRP pre-clinical laboratories, Brigham and Women's Hospital, Dana-Farber Cancer Institute
    BIOGRAPHY

Abstract

Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challenging task, especially when the position and type of mutation are unknown. We describe simple methods for enrichment of mutations in a sample, such that downstream detection or sequencing of mutations is facilitated. Using COLD-PCR, the sensitivity of almost all mutation detection methods is improved including targeted sequencing, digital PCR, and high resolution melting. Learning objectives include the adaptation of COLD-PCR to boost the mutation detection sensitivity for all these established platforms; and practical tips to get the most out of the combined technologies.


Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 13, 2022 9:00 AM +08
OCT 13, 2022 9:00 AM +08
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
AUG 10, 2022 10:00 AM PDT
AUG 10, 2022 10:00 AM PDT
Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
C.E. CREDITS
Date: October 25, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) Candida auris is a multi-drug resistant yeast that continues to be a global threat for infection and transmission in h...
OCT 01, 2015 12:00 PM PDT

Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR



Show Resources
Loading Comments...
Show Resources