Determination Of Cell Surface Markers On Circulating Immune Cells As Biomarkers For Disease Monitoring Using Spectral Flow Cytometry

Sponsored by: Cytek Biosciences

20 +25pts

Speaker

Event Date & Time

Date: July 16, 2025

Time: 8:00 AM (PDT), 11:00 AM (EDT), 5:00 PM (CEST)

Abstract

The identification of reliable biomarkers from peripheral blood is crucial for improving disease diagnosis and monitoring. Spectral flow cytometry has emerged as a powerful tool for high-dimensional deep immune profiling, offering a comprehensive view of circulating immune cell phenotypes with enhanced resolution.

We employed spectral flow cytometry to analyze peripheral blood immune cell populations in patients affected by diverse inflammatory and infectious conditions, including COVID-19 and Giant Cell Arteritis (GCA). Through multi-parametric profiling, we identified distinct cell surface markers and phenotypic shifts associated with disease severity and progression. Our COVID-19 study confirmed that non-classical monocytes and immature neutrophils expressing low CD10 and CD101 correlate with disease severity, while calprotectin expression and monocyte subsets serve as key predictive markers. Our investigation into GCA led to the discovery of a unique neutrophil signature characterized by CD62L and CD68 overexpression, potentially offering a blood-based diagnostic tool. Integration with scRNA-seq data further validated surface targets and cytokine signatures at the protein level.

Our findings underscore the value of spectral flow cytometry for translational immunology research, bridging fundamental insights and clinical application to develop robust, non-invasive biomarkers for complex immune-mediated diseases.

Key topics discussed in this webinar will include:

Immune profiling to identify surface markers, cell populations, and phenotypic shifts associated with inflammatory and infectious conditions
Confirmation and validation of reliable biomarkers for improving disease monitoring
The value of spectral flow cytometry for translational immunology research

Who should attend:

Researchers interested in biomarker discovery for inflammatory and infectious conditions
Scientists considering using spectral flow cytometry for translational research
Anyone interested in flow cytometry applications

For Research Use Only. Not intended for use in diagnostic procedures.

Webinars will be available for unlimited on-demand viewing after live event.

Determination Of Cell Surface Markers On Circulating Immune Cells As Biomarkers For Disease Monitoring Using Spectral Flow Cytometry

Dr. Aymeric Silvin