MAR 27, 2020 11:00 AM PDT

Determination of Full, Partial, and Empty Capsid Ratios for Adeno-Associated Virus (AAV) Analysis

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Applications Scientist, SCIEX CE & BioPharma
    Biography
      Tingting Li obtained her MS degree at Rutgers University in Chemistry. Tingting worked in R&D and QC labs as Scientist in pharmaceutical companies for about 4.5 years on analytical method development and validation in NJ, US and worked in R&D lab as lab manager in NGS (Next generation sequencing) industry in China for about 1 year. Tingting Li joined Beckman Coulter (Shanghai, China) as Senior Product Specialist supporting the CE product line since 01/01/2013, then transferred to Sciex (Shagnhai, China) as a member of CE Separation team since 01/01/2014. Tingting moved to United States and joined application team in Sciex as a contractor in Sciex-Brea from 08/2017 for about half a year and then was hired as Senior Biopharma Application Development Scientist in Biologics team in Sciex (Brea) since 03/2018.

    Abstract
    DATE:  March 27, 2020
    TIME:   11:00am PT, 2:00pm ET
     
    Adeno-associated virus (AAV) is one of the most commonly used delivery vehicles in gene therapy development. However, the presence of product related impurities such as empty and partial AAV capsids co-produced during the manufacturing of AAV vectors could affect the efficacy and safety of AAV products. There are multiple technologies being used concurrently for determining the ratios of these empty or partially filled capsids along with the full AAV. However, these traditional methodologies have their own set of challenges and hence drive a need for an easy to optimize method that, using minute amounts of sample, delivers high resolving power for the determination of empty/full capsid ratios, in about an hour.
     
    This webinar demonstrates a robust capillary isoelectric focusing (cIEF)-based method for AAV full and empty capsids analysis. The results show excellent resolution between full and empty capsid as well as potential partial capsid peaks for determination of their ratios. The pI profiles can be determined and used for AAV identification. The results from this methodology correlate well with orthogonal approaches such as anion exchange high performance liquid chromatography (AEX-HPLC). The sample analysis time for this method is rapid, less than 1 hour per sample. The analysis is performed on a well validated and an automated cIEF based platform to obtain reliable and reproducible results across serotypes.
     
    Learning Objectives:
    • Learn how to distinguish between full, partial, and empty AAV capsids for multiple serotypes with an easily optimized method
    • Discover the high resolving power of separating full and empty AAVs with very small pI differences (<=0.1 pH unit)
    • Realize rapid analysis times with less than 1 hour per sample
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

    Show Resources
    You May Also Like
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    Loading Comments...
    Show Resources
    Attendees
    • See more