NOV 08, 2021 9:00 AM PST

Discovering Unforeseen Protein Assemblies with Mass Photometry

Sponsored by: Refeyn

Event Date & Time
Date:  November 08, 2021
Time: 9:00am (PST),  12:00pm (EST), 6:00pm (CET)
Many enzymes form homomeric complexes comprised of subunits encoded by the same gene. Assembly into homomeric complexes can build new active sites and enable particular types of allosteric regulation. Based on the many experimental structures of oligomeric proteins, sets of symmetry rules have been proposed and are thought to yield all conceivable geometries of homomers. Here we report the discovery of a citrate synthase that forms a wholly unprecedented kind of homomeric assembly not predicted by known symmetry rules. It closely resembles a famous deterministic fractal - the Sierpiński triangle.
Molecular fractal assemblies have been a popular target in engineering efforts e.g. using small molecules that assemble on chiral surfaces, but have never been observed in nature or in solution. Using maximum likelihood phylogenetics, we show that this assembly evolved very recently from simpler dihedral hexamers within the cyanobacteria. Using mass photometry, we characterize the interfaces and show that the assembly can be dissociated in the presence of one of the enzyme's substrates or upon changes in pH, suggesting that it may represent a form of allosteric regulation. Our findings expand the space of known protein assemblies to self-terminating fractal-like complexes of different sizes.

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