DNASTAR offers an integrated suite of software for accurate assembly, alignment, and analysis of sequence data from all major next-generation sequencing platforms, supporting key workflows including targeted resequencing and variant detection. The DNASTAR gene panel workflow supports all gene panel sets from Illumina and Ion Torrent, as well as custom gene panel data sets. The efficiency of variant detection in gene panels is determined by multiple factors, including specificity of primers and probes used for gene panel design; efficiency of the sequencing technology; accuracy of assembly; accuracy of variant calling; and stringency of variant filters. When gene panel sequencing data is used in conjunction with a validated variant data set, DNASTAR software can calculate variant calling sensitivity, specificity, and accuracy. By utilizing this workflow, users can validate their entire gene panel assembly and analysis process and the speed and accuracy of DNASTAR software.