AUG 24, 2021 11:00 AM PDT

EAD easy as 1-2-3: Making characterization of complex biotherapeutics routine

Sponsored by: Sciex
C.E. Credits: P.A.C.E. CE Florida CE
Speakers

Abstract
Date:  August 24, 2021
Time: 11:00am (PDT),  2:00pm (EDT)
 
Electron-based dissociation mechanisms have shown great promise for advanced characterization of biomolecules. However, routine adoption has been limited due to poor fragmentation efficiency, need for tuning and lack of reproducibility. To unleash this promise, SCIEX has recently released the ZenoTOF 7600 system mass spectrometer that includes electron-activated dissociation (EAD). EAD is unique because the cell produces an intense, free electron beam that can be used to efficiently fragment several different compound classes. As a result, the benefits of free electron fragmentation can be realized within peptide mapping experiments on LC timescales. EAD data can be processed and annotated using Protein Metrics, Inc. ByosTM software thereby enabling advanced characterization of various protein biotherapeutics within an automated and routine workflow. In this webinar, we will describe the underlying technology of EAD within the ZenoTOF 7600. We will then share examples of EAD fragment ion spectra processed using ByosTM for the site specific determination of glycosylation, characterization of disulfide bonded peptides, and discrimination of amino acid isomer pairs like isoaspartic acid from aspartic acid and leucine from isoleucine. Finally, we will showcase an example of in depth characterization of an antibody drug conjugate digest.
 
Learning Objectives
  • Outline how electron-based fragmentation offers complimentary data to traditional CID data for advanced characterization of protein therapeutics
  • Identify how EAD enables routine implementation of electron-based fragmentation to more efficiently characterize complex protein therapeutics
  • Recognize how Protein Metrics ByosTM Software offers a simple, yet thorough solution for processing EAD data to enable lab users at all levels, and provide actionable information to managers.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
NOV 09, 2022 8:00 AM PST
C.E. CREDITS
NOV 09, 2022 8:00 AM PST
Date: November 09, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) The field of cell and gene therapy is rapidly growing. In particular, the use of lentiviruses in CAR-T applications is...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
Loading Comments...
Show Resources