NOV 01, 2022 7:00 AM PDT

Eavesdropping on Biological Processes with Multimodal and Multiplex Imaging

Sponsored by: Leica Microsystems
C.E. Credits: P.A.C.E. CE Florida CE

Event Date & Time
Date:  November 01, 2022
Time: 7:00am (PDT),  10:00am (EDT), 3:00pm (CET)
Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex events ranging from embryonic development to disease processes. However, imaging approaches are challenged by unavoidable tradeoffs between spatial resolution, temporal resolution, field of view and the limited photon budget.
We are attempting to advance this tradeoff by constructing faster and more efficient light sheet microscopes that maintain subcellular resolution. Our two-photon light-sheet microscope combines the deep penetration of two-photon microscopy and the speed of light sheet microscopy to generate images with more than ten-fold improved imaging speed and sensitivity. This combination of attributes permits 4D cell and molecular imaging with sufficient speed and resolution to generate unambiguous tracing of cells and signals in intact systems.
To increase the 5th Dimension, the number of simultaneous labels, we are refining new multispectral image analysis tools that exceed the performance of our previous work on Linear Unmixing by orders of magnitude in speed, error propagation and accuracy. hese new analysis tools permit rapid and unambiguous analyses of multiplex-labeled specimens.
In parallel, we have refined label-free approaches so that imaging and sensing can be extended to patient-derived tissues and even human subjects. The low concentrations and low sensitivity of the techniques can make single cell approaches challenging. We are refining fluorescence lifetime approaches (FLIM), combining it with multispectral tools to optimize intravital imaging in these challenging settings.
Combined, these imaging and analysis tools offer the multi-dimensional imaging required to follow key events in intact systems, and allow us to use noise and variance as experimental tools rather than experimental limitations.
Learning Objectives
  • Explain how biological imaging involves tradeoffs between resolution, speed, depth and field of view.
  • Describe how multiplex tools can increase the efficiency and the number of labels that can be imaged.
  • Demonstrate how fluorescence lifetime is a powerful but underused tool for intrinsic and extrinsic labels.
  • Discuss how phasor approaches can offer more efficient processing, especially in low light conditions.
Webinars will be available for unlimited on-demand viewing after live event.
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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