JUN 20, 2019 08:00 AM PDT

Use of Echo Acoustic Liquid Handling in Functional Genomic Screening of Retinal Ganglion Cells

SPONSORED BY: Labcyte
Speakers
  • Assistant Professor, Department of Ophthalmology, University of California, San Diego
    Biography
      An Assistant Professor of Ophthalmology at the Shiley Eye Institute, his laboratory studies the way in which glaucoma leads to optic nerve injury, neurodegeneration and, ultimately, vision loss. Specifically, he uses high-throughput genetic screening to comprehensively characterize the genes responsible for retinal ganglion cell death and axon degeneration. His ultimate goal is to develop new small molecule- and CRISPR-based neuroprotective strategies to inhibit deleterious gene function, prevent nerve cell death and improve outcomes for patients with glaucoma. His lab was recently named one of four to be selected by the Glaucoma Research Foundation for the prestigious Catalyst for the Cure initiative.
      Prior to joining UC San Diego Health, Dr. Welsbie was an Assistant Professor at the Johns Hopkins University Wilmer Eye Institute, where he won the Shafer Prize for innovative glaucoma research from the Glaucoma Research Foundation and was named Assistant Professor of the Year (2015). He also served as the Stephen J. Ryan Assistant Chief of Service and continues to have an interest in resident and medical student education.
      Dr. Welsbie completed a residency in Ophthalmology and fellowship training in Glaucoma at the Wilmer Eye Institute. He earned his medical degree and doctorate in molecular biology from the David Geffen School of Medicine at UCLA. While there, Dr. Welsbie did pioneering work in the field of cancer that led directly to the invention of enzalutamide, the first-line treatment for advanced prostate cancer.
    • Senior Product Manager, Labcyte, Inc.
      Biography
        Iain Russell has over 16 years of experience in the Life Sciences industry and the genomics field. Iain joined Labcyte in May of last year and currently manages the Echo® Liquid Handler product line, a series of instruments that enables the non-contact transfer of nanoliter liquid volumes with extreme accuracy and precision. Prior to joining Labcyte, Iain managed a broad variety of novel technologies including microarray and qPCR related instruments and associated consumables. Iain completed his Ph.D. graduate work with a focus on gene expression at the European Molecular Biology Laboratory in Heidelberg, Germany, prior to completing a post-doc at the Massachusetts Institute of Technology in the cell cycle biology field.

      Abstract:

      DATE: Thursday, June 20, 2019

      TIME:  8:00am PDT

      Retinal ganglion cells (RGCs) are projection neurons that process and transmit visual information from the retina to the brain. In a group of diseases called optic neuropathies, these cells degenerate, leading to permanent vision loss. We have been interested in using primary mouse RGCs and human induced pluripotent stem cell (iPSC)-derived RGCs to perform functional genomic screens using chemical, siRNA and, most recently, gRNA libraries. Using the Labcyte® Echo® Liquid Handler we have developed a high-throughput method to screen in cultured RGCs. Moreover, we have developed protocols to synthesize chimeric gRNAs and leverage CRISPR technology to perform site-directed mutagenesis in both primary mouse and human iPSC-derived RGCs.

      Key Learning Objectives:

      • Understand how high-throughput screening is being used to identify neuroprotective targets

      • Learn about the workflow to conduct HTS screens in primary and stem cell-derived neurons

       

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