JUN 20, 2019 8:00 AM PDT

Use of Echo Acoustic Liquid Handling in Functional Genomic Screening of Retinal Ganglion Cells

Sponsored by: Labcyte
Speakers

Abstract

DATE: Thursday, June 20, 2019

TIME:  8:00am PDT

Retinal ganglion cells (RGCs) are projection neurons that process and transmit visual information from the retina to the brain. In a group of diseases called optic neuropathies, these cells degenerate, leading to permanent vision loss. We have been interested in using primary mouse RGCs and human induced pluripotent stem cell (iPSC)-derived RGCs to perform functional genomic screens using chemical, siRNA and, most recently, gRNA libraries. Using the Labcyte® Echo® Liquid Handler we have developed a high-throughput method to screen in cultured RGCs. Moreover, we have developed protocols to synthesize chimeric gRNAs and leverage CRISPR technology to perform site-directed mutagenesis in both primary mouse and human iPSC-derived RGCs.

Key Learning Objectives:

  • Understand how high-throughput screening is being used to identify neuroprotective targets

  • Learn about the workflow to conduct HTS screens in primary and stem cell-derived neurons

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
JUN 20, 2019 8:00 AM PDT

Use of Echo Acoustic Liquid Handling in Functional Genomic Screening of Retinal Ganglion Cells

Sponsored by: Labcyte


Show Resources
Loading Comments...
Show Resources