JUN 20, 2019 8:00 AM PDT

Use of Echo Acoustic Liquid Handling in Functional Genomic Screening of Retinal Ganglion Cells

Sponsored by: Labcyte
Speakers

Abstract

DATE: Thursday, June 20, 2019

TIME:  8:00am PDT

Retinal ganglion cells (RGCs) are projection neurons that process and transmit visual information from the retina to the brain. In a group of diseases called optic neuropathies, these cells degenerate, leading to permanent vision loss. We have been interested in using primary mouse RGCs and human induced pluripotent stem cell (iPSC)-derived RGCs to perform functional genomic screens using chemical, siRNA and, most recently, gRNA libraries. Using the Labcyte® Echo® Liquid Handler we have developed a high-throughput method to screen in cultured RGCs. Moreover, we have developed protocols to synthesize chimeric gRNAs and leverage CRISPR technology to perform site-directed mutagenesis in both primary mouse and human iPSC-derived RGCs.

Key Learning Objectives:

  • Understand how high-throughput screening is being used to identify neuroprotective targets

  • Learn about the workflow to conduct HTS screens in primary and stem cell-derived neurons

 

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LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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JUN 20, 2019 8:00 AM PDT

Use of Echo Acoustic Liquid Handling in Functional Genomic Screening of Retinal Ganglion Cells

Sponsored by: Labcyte


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