NOV 20, 2020 8:00 AM PST

Efficiency to Confidence with High Resolution Accurate Mass Spectrometers: Analyzing IGF-1

Speaker
  • Clinical Development Associate, Mayo Clinic - Clinical Mass Spectrometry Lab and Clinical Forensic and Toxicology Lab
    Biography

      Tony received his Ph.D. in 2017 from the University of Wyoming based on work with his research advisor Dr. Franco Basile. His graduate studies primarily focused on proteomic analyses of bacteria using a wide variety of mass spectrometry techniques. After graduate school, he joined Mayo Clinic's Clinical Mass Spectrometry Development Laboratory, which supports and develops tests for the Clinical Mass Spectrometry Lab and the Clinical Forensic and Toxicology lab. In his current role as Clinical Development Associate he works on testing for a wide variety of targets including proteins, peptides, vitamins, steroids, therapeutic drugs, and drugs of abuse.


    Abstract

    DATE: November 20, 2020

    TIME:   8am PDT, 11am EDT

     

    Analysis of Insulin-like growth factor-1 (IGF-1) with high-resolution accurate-mass (HRAM) mass spectrometry  offers significant advantages and allows for identification of single amino acid variants; In contrast, both normal and deleterious sequence variants might be missed by immunoassays or non-HRAM-MS methods. This webinar will highlight a robust and reliable method that was developed to identify IGF-1 variants and distinguish them by a center of mass (COM) calculation, followed by various tandem-MS activation techniques (HCD, ETD, ETciD, EThcD, UVPD) using Thermo Scientific™ Orbitrap™-based mass spectrometry systems.

     

    For General Lab Use Only - Not For Diagnostic Procedures.

     

    Learning Objectives:

    1. How to use mass spectrometry to detect single amino acid differences in IGF-1 variants.
    2. How to perform qualitative and quantitative analyses of other peptides/proteins in clinical research settings that might lend itself to the characterization of other protein variations.
    3. Demonstrate the power of high resolution to detect protein and peptide analytes in complex biological matrices.

     

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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