JUN 16, 2015 08:00 AM PDT

Employing new approaches to investigate human and mouse group 2 innate lymphoid cell responses

Speakers
  • Assistant Professor, Cornell University College of Veterinary Medicine
    Biography
      Dr. Elia Tait Wojno pursues a life-long passion for the biological sciences as an Assistant Professor at the Cornell University College of Veterinary Medicine. Elia received her PhD from the University of Pennsylvania, working with Dr. Christopher Hunter in the School of Veterinary Medicine to examine the mechanisms that promote protective immunity to the protozoan parasite Toxoplasma gondii. She went on complete a postdoctoral fellowship with Dr. David Artis in the University of Pennsylvania Perelman School of Medicine and Weill Cornell Medical College, focusing on the regulation of immune responses during helminth infection and allergic disease. As an Assistant Professor at the Baker Institute for Animal Health at Cornell University, she continues her work in dissecting immunological pathways that regulate innate immune responses following helminth parasite infection and during allergy, with a special emphasis on regulation by cytokines and prostaglandins. Her work aims to increase our understanding of how our immune system protects us from infection when it is functioning optimally, and how our immune system can cause inappropriate inflammation when it becomes dysfunctional. This work will hopefully inform efforts to develop new therapies to combat infectious diseases, particularly diseases caused by parasite infection, and to limit allergic inflammation.

    Abstract:
    Date: June 16, 2015
    Time: 8:00AM Pacific time, 11:00AM Eastern Time

    Group 2 innate lymphoid cells (ILC2s) are recently described innate immune cells that contribute to type 2 inflammation, tissue repair, and metabolic homeostasis in multiple tissues. While ILC2s play key roles in various biological processes, these cells are rare, making analysis of their phenotype and function a technical challenge. This webinar will discuss cutting-edge techniques that can be used to examine this dynamic cell population in murine models and in human tissues. A project focused on assessing the role of the prostaglandin D2 receptor, CRTH2 (chemoattractant homologous molecule expressed by Th2 cells), in ILC2 responses will be used to highlight selected technical approaches and assays that can be used to analyze ILC2 phenotype and function. Techniques that will be highlighted include multi-color flow cytometry, cell sorting approaches, single-cell RNA staining, and in vitro assays for ILC2 function. In particular, the PrimeFlow™ RNA assay, which enables simultaneous assessment of protein and RNA transcript expression on a single cell level, will be discussed. The key concepts described during the session will provide relevant information for those new to the ILC field and for investigators who are already experienced in the analysis of ILC responses.

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