FEB 25, 2015 08:00 AM PST

Evaluating Nonderivatization & Derivatization Tandem MS Methods for Multianalyte Analysis in Dried Blood Spots

Speakers
  • Senior Applications Scientist, Thermo Fisher Scientific
    Biography
      Joe Di Bussolo earned his B.S. degree in clinical chemistry at West Chester University in 1977. In 1982, he received a Ph.D. degree in biological sciences from Drexel University in Philadelphia. During those college years, Joe worked part time as a medical laboratory technologist at Children's Hospital of Philadelphia. For most of the 20 years that followed, Dr. Di Bussolo helped develop and supported various analytical instruments and software products for the PerkinElmer Corporation. In 2002, Joe joined Cohesive Technologies as an applications scientist. Cohesive was acquired in 2007 by Thermo Fisher Scientific and Joe currently works under the Thermo umbrella as a senior scientist training and collaborating with customers and managing the Applications Laboratory at West Chester University of Pennsylvania. He is also an honorary faculty member and advisor in the University's Pharmaceutical Product Development program.

    Abstract:

    Date:  Wednesday, February 25, 2015
    Time(s):  08:00 PST, 11:00 EST, 16:00 UK, 17:00 CET


    Flow injection tandem mass spectrometry (FIA-MS/MS) has been frequently used to analyze amino acids (AA), acylcarnitines (AC), and succinylacetone (SUAC) in dried blood spots (DBS) for inborn errors of metabolism (IEM) research. While butyl esterification method (derivatization) is routine, it is possible to detect AAs, ACs, and SUAC as their native free acids (nonderivatization). In this webinar, we will discuss the research regarding evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer. The use of meta-calculation software for post-analytical data processing in research will also be discussed.


    Who Should Attend: due at project initiation


    Researchers who are interested in:



      • Inborn errors of metabolism (IEM) research

      • Quantitation of amino acids and acylcarnitines

      • Selected reaction monitoring (SRM) method development and verification using triple quadrupole mass spectrometer

      • Streamlining post-analytical data processing

      • Nonderivatization vs. derivatization sample preparation method comparison for IEM research


    Key Learning Objectives: due at project initiation


    What you will learn:



      • Benefits of tandem mass spectrometry in inborn errors metabolism (IEM) research

      • Workflow of using tandem mass spectrometry for analysis of AAs, ACs, and SUAC in dried blood spots (DBS)

      • Challenges of handling huge quantities of data generated from tandem MS analysis

      • Discuss a meta-calculation software for post-analytical data processing

      • Discuss research results of evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer


    For research use only. Not for use in diagnostic procedures.


    Show Resources
    You May Also Like
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 18, 2019TIME: 8:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Loading Comments...
    Show Resources