FEB 25, 2015 08:00 AM PST

Evaluating Nonderivatization & Derivatization Tandem MS Methods for Multianalyte Analysis in Dried Blood Spots

  • Senior Applications Scientist, Thermo Fisher Scientific
      Joe Di Bussolo earned his B.S. degree in clinical chemistry at West Chester University in 1977. In 1982, he received a Ph.D. degree in biological sciences from Drexel University in Philadelphia. During those college years, Joe worked part time as a medical laboratory technologist at Children's Hospital of Philadelphia. For most of the 20 years that followed, Dr. Di Bussolo helped develop and supported various analytical instruments and software products for the PerkinElmer Corporation. In 2002, Joe joined Cohesive Technologies as an applications scientist. Cohesive was acquired in 2007 by Thermo Fisher Scientific and Joe currently works under the Thermo umbrella as a senior scientist training and collaborating with customers and managing the Applications Laboratory at West Chester University of Pennsylvania. He is also an honorary faculty member and advisor in the University's Pharmaceutical Product Development program.


    Date:  Wednesday, February 25, 2015
    Time(s):  08:00 PST, 11:00 EST, 16:00 UK, 17:00 CET

    Flow injection tandem mass spectrometry (FIA-MS/MS) has been frequently used to analyze amino acids (AA), acylcarnitines (AC), and succinylacetone (SUAC) in dried blood spots (DBS) for inborn errors of metabolism (IEM) research. While butyl esterification method (derivatization) is routine, it is possible to detect AAs, ACs, and SUAC as their native free acids (nonderivatization). In this webinar, we will discuss the research regarding evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer. The use of meta-calculation software for post-analytical data processing in research will also be discussed.

    Who Should Attend: due at project initiation

    Researchers who are interested in:

      • Inborn errors of metabolism (IEM) research

      • Quantitation of amino acids and acylcarnitines

      • Selected reaction monitoring (SRM) method development and verification using triple quadrupole mass spectrometer

      • Streamlining post-analytical data processing

      • Nonderivatization vs. derivatization sample preparation method comparison for IEM research

    Key Learning Objectives: due at project initiation

    What you will learn:

      • Benefits of tandem mass spectrometry in inborn errors metabolism (IEM) research

      • Workflow of using tandem mass spectrometry for analysis of AAs, ACs, and SUAC in dried blood spots (DBS)

      • Challenges of handling huge quantities of data generated from tandem MS analysis

      • Discuss a meta-calculation software for post-analytical data processing

      • Discuss research results of evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer

    For research use only. Not for use in diagnostic procedures.

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