DEC 03, 2019 9:00 AM PST

Examination of Microglia at Single Cell Resolution in Health and Disease

Speakers

Abstract

DATE: December 3, 2019

TIME:  9:00am PST

Microglia are the brain's resident immune cells and play key roles in normal development, maintenance of homeostasis, and disease. Using a combination of single cell sequencing and RNAscope we have uncovered novel populations of microglia in both early post-natal development, injury, aging, and neurodegeneration. We have also found that microglia are uniquely sensitive cells and require great care when performing both scRNA-seq and smFISH/RNAscope to ensure accurate results.

 

Learning Objectives:

  • Discuss how Spatial Information from RNAscope is the key component of single cell studies to both localize populations spatially and validate sequencing results.
  • Describe how RNAscope verifies artifactual gene expression observed in scRNA-seq when cells isolated under improper conditions
  • Summarize how HiPlex assay enables significantly more accurate localization of specific cell states in situ

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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DEC 03, 2019 9:00 AM PST

Examination of Microglia at Single Cell Resolution in Health and Disease


Specialty

Molecular Biology

Immunology

Cell Biology

Neuroscience

Biotechnology

Genetics

Cell Culture

Cancer Research

Gene Expression

Molecular Diagnostics

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