DEC 03, 2019 9:00 AM PST

Examination of Microglia at Single Cell Resolution in Health and Disease

Speakers
  • Postdoctoral Fellow - Laboratory of Dr. Beth Stevens, F.M. Kirby Neurobiology Research Center, Boston Children's Hospital/Harvard Medical School
    Biography
      Samuel Marsh (Ph.D.) is postdoctoral fellow in the lab of Dr. Beth Stevens at Boston Children's Hospital/Harvard Medical School. Dr. Marsh received his Ph.D. at the University of California, Irvine exploring role of the immune system in Alzheimer's disease. His current work in the Stevens lab is focused on better understanding microglial function and dysfunction in normal healthy brain and Alzheimer's disease in using single cell sequencing technology.
    • Research Manager, Applications Advanced Cell Diagnostics, a Bio-Techne Brand
      Biography
        Dr. Courtney Anderson manages the research applications group at Advanced Cell Diagnostics, a Bio-Techne brand, in Newark, California. For over the last 4 years Dr. Anderson has been managing research projects in the ACD laboratories and with collaborators from around the globe to support novel scientific applications of the RNAscope technology. Prior to joining ACD, Dr. Anderson completed her postdoctoral studies in metabolic biology at the University of California Berkeley. Dr. Anderson received her Ph.D. in molecular and developmental biology from the University of California San Francisco (UCSF) and her B.A. in human biology from Brown University.

      Abstract

      DATE: December 3, 2019

      TIME:  9:00am PST

      Microglia are the brain's resident immune cells and play key roles in normal development, maintenance of homeostasis, and disease. Using a combination of single cell sequencing and RNAscope we have uncovered novel populations of microglia in both early post-natal development, injury, aging, and neurodegeneration. We have also found that microglia are uniquely sensitive cells and require great care when performing both scRNA-seq and smFISH/RNAscope to ensure accurate results.

       

      Learning Objectives:

      • Discuss how Spatial Information from RNAscope is the key component of single cell studies to both localize populations spatially and validate sequencing results.
      • Describe how RNAscope verifies artifactual gene expression observed in scRNA-seq when cells isolated under improper conditions
      • Summarize how HiPlex assay enables significantly more accurate localization of specific cell states in situ

       

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