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MAR 11, 2020 1:30 PM PDT

Examining the effects of sonication on alpha synuclein pre-formed fibrils (PFFs)

Presented at: Neuroscience 2020
Sponsored by: StressMarq Biosciences
C.E. Credits: Florida CE P.A.C.E. CE
Speaker

Abstract

Neurodegenerative disease research can be considerably accelerated by using fibrillar or oligomeric constructs of prion-like proteins in in vitro or in vivo studies. These exist in a variety of forms, and are able to generate different outcomes depending on how they are prepared. The most popular constructs are pre-formed fibrils (PFFs) of certain proteins, including Alpha Synuclein and Tau, relevant in Parkinson’s and Alzheimer’s Diseases.  However, despite being popular in this regard, their preparation – requiring sonication – has not been extensively studied from a phase perspective. In this discussion we examine current best practices and provide direction of why sonication is important and how it affect pre-formed fibrils.


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