MAR 25, 2021 8:00 AM PDT

Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles

Speaker
  • Senior bioanalytical chemist, National Institute of Standards and Technology (NIST)
    Biography

      Paul DeRose is a physical/analytical chemist who came to NIST as a National Research Council Postdoctoral Fellow in 1996. Before coming to NIST, he earned his PhD in chemistry from the University of Pennsylvania (Philadelphia, PA) where he studied molecular excited-state dynamics and intermolecular interactions using laser spectroscopy. As an NRC/NIST postdoc, he built a near-field scanning optical microscope (NSOM) and used NSOM and atomic force microscopy (AFM) to image thin films and cell membranes. His current research interests concern the development of fluorescence standards and methods for validation of chemical and clinical assays. His research has resulted in publications in various areas of fluorescence spectroscopy and microscopy. He has developed fluorescence standard guidelines and recommendations for ASTM, IUPAC and US Pharmacopeia. He is also the chair of ASTM E13.01.01 sub-committee on Molecular Luminescence. Paul currently leads the NIST Biochemical Science Division's project in luminescence standards development for chemical analysis and assay validation.


    Abstract

    Date:  March 25, 2021

    Time: 8:00am (PST),  11:00am (EST)

     

    Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data measurements, the collection of data from different instruments or on different days contribute to inaccuracies and incomparable data. In this presentation, we will introduce ERF (Equivalent Number of Reference Fluorophore) standards and discuss the significance and advantages of using the ERF standards for quantitative flow cytometry instead of pre-existing, value-assigned beads. The aim of this talk is to demonstrate ERF beads improve quality and quantification for modern fluorescence techniques.

     

    Learning Objectives

    • Introducing ERF beads and their use to calibrate fluorescence data
    • Understanding how standards are developed for flow cytometry
    • Benefits to using standards and data accuracy for flow cytometry, microscopy, viral and EV experiments

     

     

    Webinars will be available for unlimited on-demand viewing after live event.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    Loading Comments...
    Show Resources
    Attendees
    • See more