MAR 25, 2021 8:00 AM PDT

Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles

Speaker
  • Senior bioanalytical chemist, National Institute of Standards and Technology (NIST)
    BIOGRAPHY

Abstract

Date:  March 25, 2021

Time: 8:00am (PST),  11:00am (EST)

 

Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data measurements, the collection of data from different instruments or on different days contribute to inaccuracies and incomparable data. In this presentation, we will introduce ERF (Equivalent Number of Reference Fluorophore) standards and discuss the significance and advantages of using the ERF standards for quantitative flow cytometry instead of pre-existing, value-assigned beads. The aim of this talk is to demonstrate ERF beads improve quality and quantification for modern fluorescence techniques.

 

Learning Objectives

  • Introducing ERF beads and their use to calibrate fluorescence data
  • Understanding how standards are developed for flow cytometry
  • Benefits to using standards and data accuracy for flow cytometry, microscopy, viral and EV experiments

 

 

Webinars will be available for unlimited on-demand viewing after live event.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
Loading Comments...
Show Resources