MAR 25, 2021 8:00 AM PDT

Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles

Speaker
  • Senior bioanalytical chemist, National Institute of Standards and Technology (NIST)
    BIOGRAPHY

Abstract

Date:  March 25, 2021

Time: 8:00am (PST),  11:00am (EST)

 

Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data measurements, the collection of data from different instruments or on different days contribute to inaccuracies and incomparable data. In this presentation, we will introduce ERF (Equivalent Number of Reference Fluorophore) standards and discuss the significance and advantages of using the ERF standards for quantitative flow cytometry instead of pre-existing, value-assigned beads. The aim of this talk is to demonstrate ERF beads improve quality and quantification for modern fluorescence techniques.

 

Learning Objectives

  • Introducing ERF beads and their use to calibrate fluorescence data
  • Understanding how standards are developed for flow cytometry
  • Benefits to using standards and data accuracy for flow cytometry, microscopy, viral and EV experiments

 

 

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