Acoustic dispensing technology has allowed for compound plating to be highly customizable depending upon scientific needs. The ability to dispense compounds from a single well to any well of a destination plate has made plating drug combinations a possibility. At NCATS, we have applied acoustic dispensing technology to create drug combination matrices, grids which contain combinations of 2 compounds at variable ratios to allow for exploration of drug synergy. This technology required significant in house software development and integration with our existing robotic infrastructure. We now have applied this technology to drug repurposing projects for treating cancer, malaria, ebola virus and zika virus, which has been included in publications and provided the basis for ongoing clinical trials.