MAY 10, 2017 12:00 PM PDT

Expression of mAbs in an academic setting: from zero to hero

  • Sr. Scientist, Center for Molecular Medicine, UCSD
      Arjen van den Berg, Ph. D. is currently a Sr. Scientist at the department of Molecular Medicine. Arjen is part of a team of scientists dedicated to discovering and developing new ways to target the undruggable genome. His current focus is to produce, purify and characterize mAbs. Dr. van den Berg received his Ph.D. in Medicine from the University of Amsterdam, The Netherlands and his M.S. Biology, Utrecht University, The Netherlands.


    The development of antibody drug conjugates requires milligrams of different mAbs. During the discovery phase of this process, sequences may need to be altered or isotypes changed. Recombinant DNA technology allows for easy manipulation of IgG expression vectors and is not considered a bottle neck. The generation of stable CHO cell lines however is very time and labor consuming. In this brief presentation, I will share my experience using the Expicho system for transient expression and purification of milligram amounts of several mAbs in an academic environment.

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