FEB 27, 2018 8:00 AM PST

WEBINAR: Flow Cytometric Approach to Probiotic Cell Counting and Analysis

Speaker

Abstract

DATE: February 27, 2018
TIME:  8:00AM PST, 11:00AM EST

 

The complexity of the manufacturing process of probiotic supplements, along with increased demand and strict criteria for quality, potency and safety, in accordance to FDA regulations, necessitates that manufacturers continue to improve production processes that increase productivity and efficiency. Flow Cytometry is a viable option for a non-culture based method for enumerating cells for probiotics manufacture. Using the CytoFLEX flow cytometer with its ability to resolve small particles down to 200nm, it was possible to reliably count bacteria as small as 0.5μm. This analysis allowed to compare and contrast culture-based and flow cytometry based workflows for probiotic cell enumeration. The results obtained in this study indicate that flow cytometry is applicable for probiotic cell count assay; both dyes, SYTO9 and PI were able to identify viable and non-viable cells, providing the analyst additional information on the status of the cultures.

Learning objectives:

  • The importance of gating and threshold settings strategy
  • Comparison between traditional probiotic cell counts versus a new approach using flow cytometry method
  • Bacterial cell staining process

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUN 03, 2021 12:00 PM CST
JUN 03, 2021 12:00 PM CST
DATE: June 3, 2021 TIME: 12:00pm SGT This webinar is a virtual event that focuses on utilizing the Gibco CTS Rotea System for Cell and Gene Therapy...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
FEB 27, 2018 8:00 AM PST

WEBINAR: Flow Cytometric Approach to Probiotic Cell Counting and Analysis


Specialty

Immunology

Molecular Biology

Cell Biology

Biotechnology

Cancer Research

Microbiology

Cell Culture

Laboratory Testing

Clinical Diagnostics

Biochemistry

Genetics

Infectious Disease

Molecular Diagnostics

Dna

Clinical Chemistry

Geography

North America56%

Europe21%

Asia11%

South America6%

Africa3%

Oceania1%

Registration Source

Website Visitors57%

WAVE Newsletter Promotion18%

Email Promotions17%

Social Media Promotions4%

Website Banners2%

Client/Sponsor Promotions2%

Job Title

Research Scientist14%

Student14%

Medical Laboratory Technician14%

Facility/Department Manager9%

Lab Management7%

Educator/Faculty7%

Executive6%

Scientist6%

Post Doc6%

Medical Doctor/Specialist5%

Clinical Laboratory Scientist4%

Microbiologist2%

Biologist2%

QC/QA2%

Marketing/Sales1%

Chemist1%

Organization

Academic Institution14%

Clinical Laboratory10%

Hospital9%

Research Institute7%

Biotech Company7%

Life Science Company4%

Government3%

Medical Center2%

Industrial Company2%

Ambulatory Care1%

Distribution/sales1%

Medical School1%

Contract Research Organization (Cro)1%

Pharmaceutical Company1%

Other4%


Show Resources
Loading Comments...
Show Resources