FEB 27, 2018 8:00 AM PST

WEBINAR: Flow Cytometric Approach to Probiotic Cell Counting and Analysis

Speaker
  • Quality Control, Senior Microbiologist, VitaQuest International
    Biography
      Dharlene Tundo obtained her Bachelor of Science in Molecular Biology from Montclair State University and has over ten years of diverse experience in research and pharmaceutical industry. Dharlene contributed to the successful launch of two oncology drugs, Cyramza and Portrazza, at Lilly Pharmaceutical. Her research interest is focused on the study of Lactobacillus and Bifidobacterium species. She is dedicated to develop a method to analyze and obtain cell count differentiation on viable and non-viable bacterial cells for the manufacture of probiotic supplements, using the flow cytometric approach.

    Abstract

    DATE: February 27, 2018
    TIME:  8:00AM PST, 11:00AM EST

     

    The complexity of the manufacturing process of probiotic supplements, along with increased demand and strict criteria for quality, potency and safety, in accordance to FDA regulations, necessitates that manufacturers continue to improve production processes that increase productivity and efficiency. Flow Cytometry is a viable option for a non-culture based method for enumerating cells for probiotics manufacture. Using the CytoFLEX flow cytometer with its ability to resolve small particles down to 200nm, it was possible to reliably count bacteria as small as 0.5μm. This analysis allowed to compare and contrast culture-based and flow cytometry based workflows for probiotic cell enumeration. The results obtained in this study indicate that flow cytometry is applicable for probiotic cell count assay; both dyes, SYTO9 and PI were able to identify viable and non-viable cells, providing the analyst additional information on the status of the cultures.

    Learning objectives:

    • The importance of gating and threshold settings strategy
    • Comparison between traditional probiotic cell counts versus a new approach using flow cytometry method
    • Bacterial cell staining process

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