SEP 14, 2022 10:00 AM PDT

Flow Virometry Sorting, B cell Cloning and Evaluation - CytoFLEX SRT and Viral Research

Speakers

Abstract
Date:  September 14, 2022
Time: 10:00am (PDT),  1:00pm (EDT), 7:00pm (CEST)
 
Virus-like particles (VLPs) are nanostructures that possess diverse applications in therapeutics, immunization, and diagnostics. They consist of one or more different molecules, have the ability to self-assemble, and are virus particle mimics in that they share the same form and size. However, they lack the proper genetic material to be infectious. Recently, VLPs have garnered increased attention in relation to SARS-CoV-2 vaccination. Due to their ability to elicit an immune response, VLPs have become an attractive alternative to conventional vaccination methods. However, contaminants from host cell production are a major issue. Using a combination of flow virometry, as described by Tang et al, and nano-flow cytometry, one can isolate and characterize VLPs more efficiently using the CytoFLEX SRT benchtop cell sorter to selectively capture VLPs of interest. In addition, upon vaccination, it has become tantamount to measure immune response and the associated pathways. Within the mechanism of SARS-CoV-2 vaccines, both follicular helper T (Tfh) and follicular regulatory T (Tfr) cells have an effect on germinal center (GC) reactions. GC reactions generate antibodies and memory B cells, and are regulated by a balance of Tfh and Tfr response. In order to study this delicate response, single B cell clones from SARS-CoV-2 immunized mice were sorted into 96 well plates using CytoFLEX SRT benchtop cell sorter prior to further downstream applications. Specificity, affinity, and somatic hypermutation were assessed via BCR sequencing and ELISA.
 
Learning Objectives
  • VLPs: What are they and how are they beneficial to research?
  • The immunologic response to SARS-CoV-2 and associated pathways
  • Sorting as a research tool for vaccine research
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

Show Resources
You May Also Like
OCT 24, 2022 10:00 AM PDT
C.E. CREDITS
OCT 24, 2022 10:00 AM PDT
Date: October 24, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) The incredible success of mRNA vaccines against COVID-19 has generated a surge of interest and investment in the devel...
NOV 09, 2022 8:00 AM PST
C.E. CREDITS
NOV 09, 2022 8:00 AM PST
Date: November 09, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) The field of cell and gene therapy is rapidly growing. In particular, the use of lentiviruses in CAR-T applications is...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
SEP 28, 2022 9:00 AM PDT
SEP 28, 2022 9:00 AM PDT
Date: September 28, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) While COVID-19 continues to be the most pressing diagnostic focus for clinical laboratories, HIV remains a serious g...
JUN 29, 2022 8:00 AM PDT
JUN 29, 2022 8:00 AM PDT
Date: June 29, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) As of May 2022, there have been more than 500 million confirmed cases of COVID-19, including numerous variants. Additiona...
Loading Comments...
Show Resources