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JUN 28, 2016 11:00 AM PDT

Fundamentals of flow cytometry: From fluorescence to function

Speaker
  • Associate Director, Strategic Collaborations, Biosciences Division, Thermo Fisher Scientific
    Biography
      Jolene Bradford is an Associate Director, Biosciences Division of Thermo Fisher Scientific located in Eugene, Oregon, USA. She joined the company in 2001 and has developed numerous new reagents and assays for the flow cytometry platform.  Well versed in fluorescent assays, Jolene has provided numerous webinars and seminars on the topics, and has been an invited instructor at many flow cytometry training courses and workshops in the US and abroad.   More recently she has been involved in developing acoustic cytometry and instrumentation, and consults on applications. Prior to joining Thermo Fisher Scientific she performed clinical laboratory testing as a specialist in hematology and flow cytometry. In 2014, Jolene earned the Certified Cytometrist International Credential.

    Abstract

    Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. Flow cytometers are used in a broad range of applications including immunophenotyping, fluorescent protein detection, rare event analysis, live/dead cell testing, stem cell characterization, and more. As an introduction to fluorescence, this presentation covers the following topics: the principle of flow cytometry; components of a flow cytometer; basics of fluorescence and how to interpret a dye excitation/emission spectrum; how data is displayed; compensation principles and techniques; and the types of controls recommended. This webinar is useful for new and practiced cytometer users interested in learning the general concepts and principles behind flow cytometry.


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