APR 07, 2021 2:15 PM EDT

Gene Editing Validation with Highly-Accurate HiFi Reads

Speakers
  • Field Applications Manager, AMR East for Pacific Biosciences
    Biography

      Mike Weiand has spent almost 8 years with Pacific Biosciences within field applications support, enabling their customers and growing the technology. There he helps manage and assist customers in designing and conducting experiments to initiate and validate the use of the PacBio sequencing in their laboratories. Prior to PacBio, Weiand held research and development roles at Life Technologies and the Broad Institute of MIT and Harvard. While with Life Technologies, his focus was in R&D product development, assay formulation, and designing user workflows of the PGM and Proton systems. At the Broad Institute, he spent over 9 years in technology development and technology exploration, validating, scaling, and implementing next generation sequencing technologies in the high-throughput DNA sequencing platform.

    • Technical Application Scientist, GENEWIZ, A Brooks Life Sciences Company
      Biography

        Dr. Andrea O'Hara has over 11 years of experience in next generation sequencing. She earned her Ph.D. from the University of North Carolina at Chapel Hill in genetics and molecular biology. She is currently a technical application scientist at GENEWIZ within the NGS team.


      Abstract

      In this fireside chat, we’ll discuss how gene editing protocols can benefit from long-read sequencing at various stages to optimize and enhance vector integrity, which cannot be addressed using alternative approaches. With PacBio® Single-Molecule, Real-Time (SMRT®) sequencing, packaged genomes can be comprehensively profiled and vector integrity directly assessed without extensive preparation. Additionally, latest advances in SMRT sequencing have enabled greater accuracy of gene editing protocols, from early QC steps in AAV preps to characterization of on-/off-targets. In sum, leveraging PacBio's HiFi reads enable a much more comprehensive understanding of vector diversity, provide greater understanding of specific on-target changes, and discriminates sample heterogeneity at single-molecule resolution – allowing researchers discovery that cannot be interrogated with other sequencing methods due to read length limitations or amplification biases.


      Show Resources
      You May Also Like
      FEB 24, 2021 10:00 AM PST
      C.E. CREDITS
      FEB 24, 2021 10:00 AM PST
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      DEC 03, 2020 4:30 PM PST
      C.E. CREDITS
      DEC 03, 2020 4:30 PM PST
      This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
      JAN 21, 2021 8:00 AM PST
      JAN 21, 2021 8:00 AM PST
      Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
      NOV 19, 2020 8:00 AM GMT
      C.E. CREDITS
      NOV 19, 2020 8:00 AM GMT
      Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
      NOV 18, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 18, 2020 8:00 AM PST
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      MAR 30, 2021 8:00 AM PDT
      MAR 30, 2021 8:00 AM PDT
      DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
      Loading Comments...
      Show Resources