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APR 21, 2020 7:30 AM PDT

Novel CRISPR Knock-In Technology for the Robust Analysis of Cells and Tissue

Sponsored by: CasTag Biosciences
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Founder and CSO, CasTag Biosciences Chair and Professor of Cell Biology, Duke University
    Biography
      Scott Soderling's laboratory at Duke University is focused on unlocking the molecular composition of key neural cell structures to discover how proteins implicated in brain disorders function to govern diverse cellular properties underlying behavior. Utilizing novel proteomic, genome engineering, and neural circuit approaches developed in-house, they functionally link how perturbations to neural proteomes alter brain function relevant to neurodevelopment and psychiatric disorders. These models are being used to dissect a large numbers of candidate genes whose disruptions lead to critical perturbations in protein function underling disorders such as Autism, Intellectual Disability, and Schizophrenia.

    Abstract

    Modern biomedical research is being driven by large scale genetic and proteomic research to identify new targets for the study of disease mechanisms. While these techniques have been incredibly successful, it has been difficult to follow-up on these increasingly complex data sets at large scale with precision. CRISPR/Cas9 genome engineering has emerged in the last decade as a versatile and powerful toolkit to address target validation and functional analysis of genes of interest. However, many of the methods, especially those for knocking in foreign DNA sequences, require a high level of expertise, are limited in their efficiency, and are labor intensive to deploy. In this webinar, we will present a powerful new technology termed Homology-independent Universal Genome Engineering (HiUGE).

    Key topics we’ll cover include:

    - CRISPR/Cas9 Knock-In Mechanisms

    - Homology Directed Repair vs Non-Homology End Joining

    - HiUGE – payload libraries to simplify research workflows

    Learning Objectives:

    1. Can you utilize CRISPR Knock-In to benefit your research?

    2. What advantages does HiUGE have for CRISPR Knock-In?

    3. What applications are possible leveraging the HiUGE platform?


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