APR 27, 2020 9:00 AM PDT

Genome Integrity Analysis of Adeno-Associated Viruses (AAV) by Genome Sizing Methodology

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Staff Applications Scientist, Sciex
    Biography
      Dr. Jane Luo is a staff applications scientist in the Applied Biologics Work Flow Development group at SCIEX. She earned her Ph. D. in Biochemistry from the City University of New York, received postdoctoral training on Molecular Biology and Cell Biology at Weill Cornell Medical College and Harvard Medical School, and performed cancer research as an assistant adjunct professor at UC Irvine. In 2002, she moved to industry to develop capillary electrophoresis based products and applications.

    Abstract
    DATE:  April 27, 2020
    TIME:   9:00am PT, 12:00pm ET
     
    Recombinant adeno-associated virus (rAAV) is a small (25-nm) virus that is composed of a protein shell called a capsid and a single-stranded DNA genome containing the transgene and other structural and regulatory elements. During the process of packaging the transgene into the rAAV capsid, errors can happen leading to packaging of truncated genome as well as fragments from host cell genome and plasmid vector. Size determination of rAAV genome offers insights in assessing the transgene integrity of the rAAV.
     
    Verification of rAAV genome size has been traditionally done by denaturing agarose-gel electrophoresis and southern blot. Both are time consuming and with limited precision and resolution on size determination. In this webinar, we present a genome sizing methodology for assessing AAV transgene integrity using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). Our results indicate that this method is fast and robust, capable of separating intact and partial AAV genome as well as small size impurities with excellent resolution and repeatability.
     
     
    Learning Objectives:
    • Discover a Genome Sizing Methodology for Transgene Integrity Analysis of AAV Gene Therapies
    • Separate Intact and Partial AAV Genomes from Small Size Impurities Within 15 Minutes
    • Rapid Screening Workflow Allows for 30 Minute Analysis
    • Applicable Methods for Various AAV Serotypes with Easy Sample Prep
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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