SEP 11, 2019 10:30 AM PDT

Genomic characterization of emerging carbapenem-resistant Klebsiella pneumoniae infections at a public hospital in Lima, Peru

Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Hospital Nacional Cayetano Heredia, and Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Peru
    Biography
      I am a Peruvian microbiologist with a long-standing interest in infectious diseases affecting Latin American populations. My research training and interests lie at the interface of clinical microbiology, bioinformatics and infectious disease epidemiology. In 2015, I obtained my PhD in Molecular Microbiology at Washington University in St. Louis under the guidance of Dr. Gautam Dantas, where my thesis work lead to a research article in Nature. Later, as a Chevening Scholar, I obtained an MSc in Public Health in Developing Countries at the London School of Hygiene and Tropical Medicine. In 2017, I returned to Peru to establish my research group at Universidad Peruana Cayetano Heredia, where I had obtained my BSc degree 11 years earlier. As principal investigator of the Microbial Genomics Laboratory, I lead an interdisciplinary team of biologists, veterinarians, physicians and epidemiologists working on the genomic surveillance of bacterial pathogens relevant to Peruvian public health. I currently mentor four graduate students and six undergraduate researchers. I am currently supported by UPCH's 'return scholarship,' aimed at foreign-trained Peruvian scientists wishing to establish their research groups at UPCH. As a new investigator, I am committed to conducting rigorous science relevant to Peruvian public health and providing a supportive and nurturing environment for my team.

    Abstract

    In contrast to other countries in Latin America, Peru had been notoriously spared by the global dissemination of carbapenem-resistant Klebsiella pneumoniae (CR-Kp) until recently. Isolated cases of KPC-producing K. pneumoniae have been reported since 2013 in Lima. However, by 2017, rapid emergence of CR-Kp occurred  at a tertiary care hospital in Lima. Here, we  describe the molecular characteristics of the CR-Kp isolates recovered during this emergence. We conducted a retrospective study of the emerging cases of CR-Kp infections at a tertiary hospital in Lima, from July 2015 to December 2017. Kp was isolated from various samples and profiled using the VITEK system and standard biochemical tests. Whole genome sequencing was performed using an Illumina MiSeq instrument at 50–100x coverage and bioinformatic analysis was conducted using CLC Genomics Workbench v12 (QIAGEN Bioinformatics). De novo assemblies were generated to identify virulence and antibiotic resistance genes. Reference-based mappings were used to infer maximum-likelihood phylogenies from SNP alignments. Fifty-nine unique CR-Kp cases were identified during the study period. A significant increase in the number of cases was observed in 2017 compared to previous years. Carbapenemase genes of were detected in 40 isolates (95%);  24 (60%) were blaNDM-1, 13 (32%) were blaKPC-2 and 3 (8%) were blaIMP-16. Isolates carrying blaKPC were more prevalent during years 2015–2016 and blaNDM isolates were more prevalent during 2017 (p<0.0001). Multiple sequence types were identified carrying the KPC, NDM and various extended-spectrum beta-lactamases, suggesting that the increase in cases was due to infection from multiple carbapenem-resistant clones. We show that multiclonal expansion of CR-Kp strains has occurred at our institution and blaKPC has been replaced by blaNDM as the prevalent resistance mechanism since 2017.

    Fiorella Krapp1,2, Coralith García1,2, Karen Ocampo2, Lizeth Astocondor2, Catherine Amaro3, Pablo
    Tsukayama2,4
    1 Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru
    2 Instituto de Medicina Tropical “Alexander von Humbold”, Universidad Peruana Cayetano Heredia, Lima, Peru
    3 Hospital Nacional Cayetano Heredia, Lima, Peru
    4 Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Peru


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