JUL 15, 2021 8:00 AM PDT

The Good, the Bad and the Ugly of 45 color panel design: Building to higher dimensional flow cytometry while maintaining biological resolution

Speaker

Abstract
Date:  July 15, 2021
Time: 8:00am (PDT),  11:00am (EDT)
 
High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Fluorescent dye performance heavily impacts both the quality and the dimensionality of flow cytometry experiments. The development of fluorescent labels engineered with targeted excitation and emission spectra using the Invitrogen™ Phiton™ DNA platform has allowed us to expand the number of biological questions that can be asked in a single panel from 40 to 45 colors. However, as we push the theoretical limit of detection, data analysis increases in complexity with the combination of spectral unmixing and data deconvolution. In this tutorial, we take a deep dive into the data generated by the world's first 45 color flow experiment, examining what works and what doesn't in high dimensional flow cytometry. We will also explore the iterative process involved in developing and evaluating high parameter spectral flow cytometry panels.
 
Learning Objectives
  • Discuss nd understand the relationship between fluorophores and data quality
  • Discuss building 45 color flow cytometry experiment
  • Discuss problems with dyes for the red laser channels
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 13, 2021 7:00 AM PDT
C.E. CREDITS
OCT 13, 2021 7:00 AM PDT
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
OCT 14, 2021 9:00 AM PDT
C.E. CREDITS
OCT 14, 2021 9:00 AM PDT
Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
Loading Comments...
Show Resources