JUL 15, 2021 8:00 AM PDT

The Good, the Bad and the Ugly of 45 color panel design: Building to higher dimensional flow cytometry while maintaining biological resolution

Speaker

Abstract
Date:  July 15, 2021
Time: 8:00am (PDT),  11:00am (EDT)
 
High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Fluorescent dye performance heavily impacts both the quality and the dimensionality of flow cytometry experiments. The development of fluorescent labels engineered with targeted excitation and emission spectra using the Invitrogen™ Phiton™ DNA platform has allowed us to expand the number of biological questions that can be asked in a single panel from 40 to 45 colors. However, as we push the theoretical limit of detection, data analysis increases in complexity with the combination of spectral unmixing and data deconvolution. In this tutorial, we take a deep dive into the data generated by the world's first 45 color flow experiment, examining what works and what doesn't in high dimensional flow cytometry. We will also explore the iterative process involved in developing and evaluating high parameter spectral flow cytometry panels.
 
Learning Objectives
  • Discuss nd understand the relationship between fluorophores and data quality
  • Discuss building 45 color flow cytometry experiment
  • Discuss problems with dyes for the red laser channels
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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