APR 20, 2021 4:30 PM PDT

High-throughput clinical and environmental viral extraction and sequencing.

Sponsored by: Tecan
C.E. Credits: CEU
Speaker

Abstract

COVID-19 is a severe disease that has caused >1 million deaths in under one year. As this disease is novel, the molecular and cellular underpinnings of the progressive tissue injury are poorly understood, as is the role of direct versus indirect viral-induced injury. This study compared spatially-resolved gene expression in fixed lung tissue from autopsies of COVID-19 patients with high and low viral loads to those who suffered from other respiratory diseases – flu or non-viral acute respiratory distress syndrome (ARDS) – and normal lung tissue. We used the DreamPrep™ NAP workstation to extract RNA and DNA to profile 735 COVID-19 patients. Using normal samples as a reference, there was an enrichment of genes involved in antigen presentation and interferon response in high and low viral load patients. Between COVID-19 cohorts, there was an overall upregulation of individual interferon-stimulating genes in COVID-high patients, and an increase in collagen genes found in COVID-low patients. Pathway analysis revealed enrichment in interferon alpha/beta and gamma signaling in COVID-19 samples compared to normal, and this enrichment scaled with viral load. COVID-high patients showed enrichment in these pathways compared to flu and ARDS patients, while COVID-low patients had enrichment of collagen-related pathways compared to flu and ARDS.


Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
AUG 16, 2022 11:00 AM PDT
AUG 16, 2022 11:00 AM PDT
Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
Loading Comments...
Show Resources