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APR 22, 2020 6:00 AM PDT

Improved Genome Editing Using Single-Stranded DNA

Sponsored by: GENEWIZ
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Director of Research and Development for GENEWIZ
    Biography
      Dr. Chen serves as Associate Director of Research and Development for GENEWIZ and has been working on various projects involving NGS and gene editing services. Dr. Chen received his Ph.D. from Max Planck Institute in Germany, where his research was focused on neuroscience. He also worked as a post-doctoral researcher in Biophysics and Immunology at Swinburne University in Australia.

    Abstract

    CRISPR-Cas9 gene editing is a powerful technique that enables genetic modification with greater speed and accuracy than previous approaches. In this webinar, Dr. Chen highlights the advantages of single-stranded DNA vs double-stranded DNA for knock in experiments. He further discusses the tips for designing ssDNA, methods for synthesis and various applications of single-stranded DNA.

    Learning Objectives:

    1. Understanding the mechanism and importance of the Homology Directed Repair (HDR) pathway for CRISPR mediated gene knock-ins

    2. Understand why ssDNA is a powerful tool for gene knock-ins and why ssDNA is superior compared to dsDNA in terms of efficiency, cellular toxicity, and specificity

    3. Multiple methods of ssDNA synthesis are available; however, the nicking enzyme-based method results in optimum quality


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