MENU

Improved Neuronal Performance with the Gibco B-27 Plus Culture System

Speaker

Abstract

Human iPSC-derived neurons have increasingly become a valuable system for the study of neurological disorders.   Robust cell reprogramming and improved differentiation protocols enable scientists to generate patient-specific, disease in a dish models for disorders such as Parkinson's, Alzheimer's, and Autism, among others.  These human models tend to be flexible, scalable and maintain many of the characteristics of found in these disorders, which are key requirements for their use in mechanistic and drug discovery studies.  Further, the development of gene editing technology has spawned intense interest in the use of gene-edited, patient-specific iPSC-derived neurons in cell therapy applications for the treatment of neuro-degenerative disorders.

A critical step in generating useful iPSC-derived neurons is neuronal maturation.  During maturation neurons extend neurites to form highly connected networks, express synaptic markers, and become electrically active.  Typical maturation conditions are inefficient,  generating poorly matured neurons with low levels of functionality over extended periods of time.  Recently we developed a new neuronal neuronal maturation and maintenance system, (B-27TM Plus and NeurobasalTM Plus) and showed significantly improved neuronal survival, maturation, and functionality of primary rodent neurons compared to other culture systems.

Here we expand our studies to PSC-derived neurons, utilizing multiple human lines PSC and different approaches for neural stem cell derivation.  Diverse endpoints were used to interrogate maturation; neurite outgrowth, neuronal maturation marker expression (through quantitative imaging), and functionality through Multi-Electrode Array (MEA) analysis.  We found that human PSC-derived neurons matured in the new “Plus” system showed both accelerated neurite outgrowth and improved activity as compared to other approaches.   Additionally our studies highlight the importance of optimizing several key parameters, including extracellular matrix coating concentrations and delivery conditions for improved reproducibility and quality of stem cell derived neural cultures.


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
MAR 18, 2021 8:00 AM PDT
C.E. CREDITS
MAR 18, 2021 8:00 AM PDT
DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
JUN 03, 2021 12:00 PM CST
JUN 03, 2021 12:00 PM CST
DATE: June 3, 2021 TIME: 12:00pm SGT This webinar is a virtual event that focuses on utilizing the Gibco CTS Rotea System for Cell and Gene Therapy...

Improved Neuronal Performance with the Gibco B-27 Plus Culture System


Specialty

Research And Development

Earth Science

University

Research

Health

Geography

Asia50%

Registration Source

Website Visitors100%

Job Title

Medical Laboratory Technician100%

Organization

Manufacturer - Other50%


Show Resources
Loading Comments...
Show Resources