MAY 22, 2017 8:00 AM PDT

WEBINAR: Interactions between the Mycobiome and Bacteriome: Impact on Health and Disease

Sponsored by: Zymo Research
Speakers
  • Professor and Director
    Biography
      Dr Mahmoud Ghannoum received MSc in Medicinal Chemistry and PhD in Microbial Physiology from University of Technology in England, and an MBA from the Weatherhead School of Management at Case. Presently he is a tenured Professor and Director of the Center for Medical Mycology, Case Western Reserve University and University Hospitals Case Medical Center (UH) where he established a multidisciplinary disciplinary Center of Excellence that combines basic and translational research investigating microbes from the test tube to the bedside. He is also a fellow of the Infectious Disease Society of America and past President of the Medical Mycological Society of the Americas (MMSA). In 2016, Dr Ghannoum received the Rohda Benham Award presented for his continuous outstanding and meritorious contributions to medical mycology from the Medical Mycological Society of the Americas and he also received the Freedom to Discover Award from Bristol-Myers Squibb for his work on microbial biofilms.
    • Scientist, Zymo Research Corp.
      Biography
        Dr. Shuiquan Tang is currently a research scientist at Zymo Research. He received his Ph.D. in the Department of Chemical Engineering and Applied Chemistry from the University of Toronto. Dr. Tang joined Zymo Research in 2014, and has since been instrumental in the research and development of Zymo's microbiomics and metagenomics programs. His past experiences include work in the fields of environmental microbiology and biological process engineering, with an emphasis on bioinformatics, metagenomics and anaerobic cultivation. Most recently, Dr. Tang has been involved in the development of Zymo Research's microbiomics portfolio. He has multiple publications in different journals, including Frontiers in Microbiology, Applied & Environmental Microbiology and Philosophical Transactions of the Royal Society of London, Series B, Biological Science.

      Abstract

      DATE: May 22, 2017
      TIME: 8:00AM PDT, 11:00AM ET

      Crohn's disease (CD) results from a complex interplay between host genetic factors and endogenous microbial communities. In the current study, we used Ion Torrent sequencing to characterize the gut bacterial microbiota (bacteriome) and fungal community (mycobiome) in patients with CD and their non-diseased first degree relatives (NCDR) in 9 familial clusters living in Northern France/Belgium, and in healthy individuals from 4 families living in the same area (non-CD unrelated, NCDU). Principal components analysis, diversity, and abundance analyses were conducted and CD-associated inter- and intra-kingdom microbial correlations determined. Significant microbial interactions were identified and validated using single- and mixed-species biofilms. CD and NCDR groups clustered together in the mycobiome, but not in bacteriome.Microbiota of familial (CD, NCDR) samples were distinct from that of non-familial (NCDU) samples. Abundance of Serratia marcescens (SM), Escherichia coli (EC) was elevated in CD patients, while that of beneficial bacteria was decreased. Abundance of the fungus Candida tropicalis (CT) was significantly higher in CD compared to NCDR (P = .003), and positively correlated with levels of anti–Saccharomyces cerevisiae antibody (ASCA). Abundance of CT was positively correlated with SM and EC, suggesting these organisms interact in the gut. The mass and thickness of Triple species (CT+SM+EC) biofilm were significantly higher than single and double species biofilm.  CT biofilms comprised of blastospores, while double and triple species biofilms were enriched in hyphae. SM used fimbriae to co-aggregate or attach with CT/EC, while EC closely apposed with CT. Specific inter-kingdom microbial interactions may be key determinants in CD. Since recent research is showing that this fungal-bacterial interaction is detrimental to the host, novel prevention and treatment approaches are needed to interfere with this cooperation and restore the microbiome balance.

      Learning Objectives:

      • Attendees will learn about  the mycobiome contribute to digestive tract health and disease 
      • Attendees will learn how individual steps in their microbiomics workflow can be standardized to greatly improve the quality and reproducibility of the data generated

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