JUN 18, 2019 8:00 AM PDT

An Introduction to characterization of biomolecules using analytical ultracentrifugation

Sponsored by: Beckman Coulter
Speakers

Abstract
DATE: June 18, 2019
TIME: 8:00am PDT

Analytical Ultracentrifugation (AUC) is one of the most powerful biophysical tools used today for the characterization of biological samples ranging from small drug molecules to intact viruses, vesicles and microparticles. AUC works with biological samples in the native state and does not depend on a reporter species or custom-coated substrates which are required by techniques such as NMR, EPR, SPR and fluorescence spectroscopy. AUC separates biomolecules based upon both molecular mass and anisotropy, unlike Size Exclusion Chromatography (SEC), which separates molecules based only on their hydrodynamic volume. Furthermore, AUC is not just useful for characterizing the oligomerization/aggregation state of biological samples, but also to quantify interactions between different species.

In this talk, we will start with the principles of analytical ultracentrifugation and take a tour through the technology behind modern AUC, including UV/Vis absorbance mode and interference mode signal detection, and look at experiment design and setup. Following, we will address the different types of AUC experiments (equilibrium and velocity), compare and contrast their merits with sample data, and touch upon the principles of data processing (to be covered in-depth in a subsequent webinar). Finally, we will explore a variety of applications with a focus on the unique advantages that AUC brings to the study of various biotherapeutics, polymers, nanoparticles, and others – and how AUC compares to and complements other analytical techniques.

 

Learning Objectives:

  • Become familiar with the principles of analytical ultracentrifugation, the technology behind modern AUC.
  • Learn the fundamentals of how to design and setup experiments.
  • Learn about the different types of AUC experiments (equilibrium and velocity), compare and contrast their merits.
  • Learn the basic principles behind AUC data analysis.
  • Discover the unique advantages that AUC brings to the study of various biotherapeutics, polymers, nanoparticles, etc.

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 

 

 

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

 


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JUN 18, 2019 8:00 AM PDT

An Introduction to characterization of biomolecules using analytical ultracentrifugation

Sponsored by: Beckman Coulter

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