SEP 02, 2020 10:30 AM SGT

Connecting the dots between personalized and precision medicines

Speaker

Abstract

The general focus of my lab is to characterize at the molecular level, genome editing proteins derived from the bacterial CRISPR-Cas system and apply them for reversing disease causing mutations. Using biophysical tools, we dissect the mechanism of action of Cas9 proteins with respect to nucleic acid interrogation and use this knowledge to design editors with high gene targeting specificity. We combine biochemical tools for producing recombinant Cas9 proteins, induced pluripotent stem cell (iPSC) technology for studying their efficacy in patient cells and single molecule experiments for evaluating their targeting specificity. Based on this broad theme, there are two key areas where I carry out research on:

1) We have extensively characterised a TypeII Cas9 from Francisella novicida (FnCas9) and demonstrated its ability in genome editing of the sickle cell anemia mutation in patient derived iPSCs and HSCs. FnCas9 is highly specific in target interrogation and produces negligibe background insertions/deletions in patient Hematopoietic stem cells. Currently we are working towards completing pre-clinical studies on FnCas9

2) We have repurposed the high specificity of FnCas9 towards a Point of Care diagnostic assay for detecting nucleotide variants in a target. This can perform with minimum equipment and technical know-how and is being currently deployed for rapid and accurate diagnosis of COVID- 19 positive cases. By tying up with industries, we are scaling up the production of assay components to enable testing on a large scale during the ongoing pandemic.


Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
SEP 16, 2021 8:00 AM PDT
C.E. CREDITS
SEP 16, 2021 8:00 AM PDT
Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
DEC 07, 2021 10:00 AM PST
C.E. CREDITS
DEC 07, 2021 10:00 AM PST
Date: December 07, 2021 Time: 10:00am (PST) The RNAscope™ technology allows high sensitivity and spatial resolution providing pivotal single-cell information to gain better insights in...
JUN 24, 2021 8:00 AM PDT
C.E. CREDITS
JUN 24, 2021 8:00 AM PDT
Date: June 24, 2021 Time: 8:00am (PDT), 11:00am (EDT) Cardiovascular disease is a leading health problem, affecting almost 30% of individuals in the developed world, and comprises a wide ran...
Loading Comments...
Show Resources