MAY 13, 2015 9:00 AM PDT

Keynote: Next-Generation RNA-Seq Workflows and Analysis

Speaker
  • Vice President and Distinguished Scientist Illumina
    Biography
      Dr. Schroth is currently a Vice President and Distinguished Scientist at Illumina where he directs the Core Applications Group based in San Diego.

      He obtained his Ph.D. in biochemistry from the University of California at Davis and has been working in the field of next-generation sequencing (NGS) for more than a decade as part of Illumina (and Solexa).

      In his research Dr. Schroth uses NGS to study genomics, gene structure, expression and regulation and applies this to genomic projects in the fields of cancer, microbiology and infectious disease.

      Over the course of his career Dr. Schroth has published over 90 peer reviewed research papers and holds 17 U.S. patents.

    Abstract

    A well done RNA-Seq experiment can provide the most comprehensive, accurate and unbiased way to study gene expression, alternative splicing, RNA variation and RNA structure. The past few years have seen amazing technological advances that have led to a wide range of improvements in the RNA-Seq experimental process. Next-Generation RNA-Seq studies are unbiased and accurate, yet sensitive enough to work well with low amounts of total RNA (even if the RNA is highly degraded or comes from FFPE samples). These new methods are also less expensive, require less hands-on-time, and are easier to perform than ever before. Finally the data analysis bottleneck associated with RNA-Seq studies has been completely removed with the advent of pipelines that take full advantage of massively parallel cloud computing resources. In this talk I will review the state-of-the-art in RNA-Seq experimental design, library prep, sequencing and data analysis.


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