OCT 05, 2016 12:00 PM PDT

Label-free FTIR imaging to distinguish malignant from non-malignant tissues

Speaker

Abstract

FTIR spectroscopic imaging is an emerging tool for label-free, non-destructive characterization of tissues. The pathological annotation of tissue can be performed in an automated and objective manner, consistent with existing pathology sample workflows with high sensitivity and specificity often over 95% respectively. The approach is applied to fresh frozen or formalin fixed paraffin embedded thin sections of tissue biopsies (e.g. surgical resections or tissue microarrays) with spectral databases for colon, bladder and lung having been established to characterize tissue types. As part of working towards identifying differences between malignant and non –malignant samples as part of a research tool we were able to differentiate between the predictive subtypes of the adenocarcinoma in lung cancer tissue. It was also possible to grade colon cancer tissue as per current classification methods using FTIR imaging. In recent not yet published studies, this label-free technique was combined with laser-microdissection. Thereby the heterogeneity of tumor samples is overcome and homogenous cancer samples are provided for proteome-analysis and Next-Generation-Sequencing allowing for more accurate and sensitive analysis.   This approach is applied to differentiate subtypes of mesothelioma cancer and corresponding biomarkers were identified.
 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
OCT 05, 2016 12:00 PM PDT

Label-free FTIR imaging to distinguish malignant from non-malignant tissues



Show Resources
Loading Comments...
Show Resources