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NOV 15, 2018 1:00 PM PST

LIVE TRAINING: Transfection using the Neon Transfection System

Speaker
  • Scientist I, Thermo Fisher Scientific
    Biography
      Jackie completed her undergraduate degree BSc (Biochemistry) at UCLA. After her degree, she spent a year at a biotech/computer science startup in South San Francisco, California and then joined Thermo Fisher Scientific as a Scientist on the Custom Driven Biology Services Team in Carlsbad, California, where she is responsible for many projects involving the hiPSC and neuronal product portfolios.

    Abstract

    The Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation culture conditions are essential for increased cell survival for downstream applications. This live training will review protocols involving Neon electroporation of hiPSC and post electroporation culture, using delivery of CRISPR/Cas9-mediated genome editing tools as the example.


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