Local and Acute Optogenetic Control of Microtubule Dynamics in Live Cells

Speaker

Abstract

Microtubules (MTs) are dynamic cytoskeleton polymers forming a polarized intracellular network that provides structural support, facilitates intracellular transport and drives chromosome segregation. To investigate how microtubules control cell dynamics with high spatial and temporal accuracy, we developed a photo-inactivated variant of the key MT plus-end adaptor EB1, that allows local and acute manipulation of MT growth dynamics by controlling the recruitment of EB-dependent plus end tracking proteins (+TIPs) to MT ends. Blue light-induced rapid and reversible dissociation of the +TIP complex from MTs results in a MT growth rate decrease and induces depolymerization of cell edge associated MTs (van Haren et al. 2018, Nat. Cell Biol. 20, 252–261). I will discuss our development and validation of this optogenetic tool and results supporting independent roles of +TIP complexes and the canonical MT polymerase XMAP215/CKAP5 in promoting physiological MT growth rates in cells, as well as ongoing work investigating the consequences of local modulation of MT growth on neuron morphogenesis.


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
JUN 24, 2021 10:00 AM PDT
C.E. CREDITS
JUN 24, 2021 10:00 AM PDT
Date: June 24, 2021 Time: 10:00am PDT The Chan Zuckerberg Initiative (CZI) was founded to help solve some of society’s toughest challenges— from eradicating disease and improving...
Loading Comments...
Show Resources