MAY 19, 2020 8:00 AM PDT

Looking Beyond PCR: Isothermal Amplification

Speakers
  • Product Manager, Custom Molecular Biology Products Management Group, Thermo Fisher Scientific
    Biography
      Dr. Agne Alminaite has studied Molecular Biology in Vilnius University (Lithuania) and earned her Ph.D. in Virology from the Faculty of Medicine, University of Helsinki (Finland). She moved to work as a scientific consultant for the International Potato Center in Lima, Peru. In 2011 Agne joined Thermo Fisher Scientific and after several years in R&D advanced to the role in product management. She is currently the Product Manager for Custom Molecular Biology Products, taking care of the portfolio that includes all the isothermal amplification enzymes, Phi29, the Phi29 polymerase mutant - Equiphi29 and the Bsm DNA polymerase as well as broad spectrum of enzymes for DNA and RNA modification, NGS and cloning.
    • Lead R&D Manager, NGS Library Preparation and Protein In Vitro Evolution Group, Thermo Fisher Scientific
      Biography
        Dr. Remigijus Skirgaila earned his Ph.D. in Biochemistry from Vilnius University (Lithuania), did a postdoc in University of Zurich (Switzerland) and joined Thermo Fisher Scientific in 2005. He is currently the lead Senior R&D Manager for NGS library preparation and protein in vitro evolution. He has led the development of the market leading reverse transcriptases Superscript IV and Maxima H-, as well as the Platinum SuperFi and Platinum Taq II PCR enzymes. In addition to RT-PCR enzymes Remigijus was also involved in the development of new generation phi29 polymerase named as Equiphi29, which was specifically designed for fast and uniform whole genome amplification (WGA) by multiple displacement amplification (MDA).

      Abstract
      DATE:  May 19, 2020
      TIME:  8:00am PT, 11:00am ET
       
      Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a variety of methods, including techniques that utilize isothermal amplification. Several methods of isothermal amplification are available – each with advantages and challenges. In this webinar, we will cover two methods, WGA and LAMP, that are commonly used in today’s laboratories.
       
      Whole genome amplification (WGA) is a valuable tool to produce large quantities of genomic DNA from limited samples or even single cells. Using a WGA technique called multiple strand displacement amplification (MDA), we will show how the challenges of low DNA input can be overcome using a next generation enzyme, EquiPhi29 DNA Polymerase, an engineered phi29 DNA Polymerase mutant. We will demonstrate that uniform amplification can be obtained from single cells, while leveraging time-saving protocols.
       
      Another method of isothermal amplification, loop-mediated isothermal amplification (LAMP), is a rapid method for detecting low amounts of DNA. This technique eliminates the need for a thermal cycler and is increasingly used in rugged field settings for the rapid diagnosis of plant pathogens or infectious disease agents like malaria, Zika, or tuberculosis. We will show how LAMP may be used in specific molecular diagnostics applications.
       
      Learning Objectives:
      • Understand how the challenges of amplification from low input DNA, including single cells, can be overcome
      • Discover why more researchers are using a next generation enzyme, EquiPhi29 DNA Polymerase, to obtain uniform DNA amplification by MDA
      • Learn about the utility of LAMP for molecular diagnostic assay development
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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