July 26 8am PLT, 11am CTT, 12pm JST, 1pm AET

Looking Beyond PCR: Isothermal Amplification Webinar


Event Date & Time
Date: July 25
Time: 7am PT, 3pm BST, 4pm CEST
Date: July 26
Time: 8am PLT, 11am CTT, 12pm JST, 1pm AET
Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have emerged as effective solutions. This webinar focuses on two commonly used methods: whole genome amplification (WGA) and loop-mediated isothermal amplification (LAMP).
WGA, particularly employing the multiple strand displacement amplification (MDA) technique, allows the generation of large quantities of genomic DNA from limited samples or even single cells. The webinar highlights the utilization of EquiPhi29 DNA Polymerase, an engineered phi29 DNA polymerase mutant, to address low DNA input challenges. Through this presentation, attendees will observe the achievement of uniform amplification from single cells while benefiting from time-saving protocols.
The webinar also explores LAMP as an affordable and rapid method for detecting minimal amounts of DNA. Notably, LAMP eliminates the requirement for a thermal cycler and is increasingly deployed in robust field settings for prompt diagnosis of plant pathogens or infectious disease agents such as malaria, Zika, or tuberculosis. The session delves into the specific applications of LAMP in molecular diagnostics, shedding light on its efficacy in diverse scenarios.
Learning Objectives
  • Outline how the challenges of amplification from low input DNA, including single cells, can be overcome
  • Discover why more researchers are using a next generation enzyme, EquiPhi29 DNA Polymerase, to obtain uniform DNA amplification by MDA
  • Explain about the utility of LAMP for molecular diagnostic assay development
Webinars will be available for unlimited on-demand viewing after live event.
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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