MENU
SEP 16, 2020 2:00 PM EDT

Mapping Tissues with In Situ Single-Cell Transcriptomics

Speaker
  • Boston Children's Hospital
    Biography
      Dr. Jeffrey Moffitt received his PhD in Physics from the University of California Berkeley under the direction of Dr. Carlos Bustamante, where he developed novel optical manipulation methods to study molecular motors. He received postdoctoral training from Dr. Xiaowei Zhuang at Harvard University, where he developed methods for transcriptome-scale imaging in single cells. In particular, he was a co-inventor of the spatially resolved single-cell transcriptomics approach known as MERFISH. Dr. Moffitt is an Investigator in the Program in Cellular and Molecular Medicine at Boston Children's Hospital and an Assistant Professor in the Department of Microbiology at Harvard Medical School. His laboratory is developing new spatially resolved methods for single-cell 'omics and using these tools to discover and map cells in a wide range of tissues. His laboratory is supported by a Pew Biomedical Scholar Award, the NIH, and the Chan Zuckerberg Initiative.

    Abstract

    Image-based approaches to single-cell transcriptomics are emerging as powerful complements to single-cell RNA sequencing, in part, because these techniques preserve the native spatial context of RNAs within cells and tissues. I will describe multiplexed error robust single-molecule fluorescence in situ hybridization (MERFISH)—a technique capable of imaging thousands of different RNAs simultaneously in fixed cells—and its use for the discovery and mapping of cell types within intact tissues.


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 03, 2020 9:00 AM PDT
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    C.E. CREDITS
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    JUN 17, 2020 1:30 PM PDT
    C.E. CREDITS
    JUN 17, 2020 1:30 PM PDT
    Understanding the complex interplay between a pathogen and the host response is important to developing effective vaccines and therapeutics. The nCounter® Analysis System and GeoMx®...
    NOV 05, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 05, 2020 7:00 AM PST
    DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
    NOV 19, 2020 8:00 AM GMT
    C.E. CREDITS
    NOV 19, 2020 8:00 AM GMT
    Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
    Loading Comments...
    Show Resources
    Attendees
    • See more