JUN 16, 2020 8:00 AM PDT

Measuring molecular interactions by multi-wavelength analytical ultracentrifugation

Speaker
  • Professor, University of Lethbridge, Department of Chemistry and Biochemistry
    Biography
      Borries Demeler started his education at the University of Montana, earning a degree in chemistry and mathematics. He then joined Prof. Ken van Holde at Oregon State University for his graduate studies, earning a PhD in biochemistry and biophysics with his work involving biophysical characterization of chromatin. It was in Dr. van Holde's laboratory that he first learned about AUC on a 30 year old Beckman Model-E instrument with UV absorbance optics and a strip chart recorder. He digitized the instrument and wrote his first version of UltraScan to analyze AUC experiments. Next, he moved to Texas to join Prof. Jeff Hansen's laboratory at the University of Texas Health Science Center in San Antonio for a postdoctoral position, where he ported UltraScan to the XLA/I instruments. After completing his postdoc, he joined the Biochemistry faculty at UTHSCSA and became director for the Center for Analytical Ultracentrifugation of Macromolecular Assemblies (CAUMA), which he still manages today. CAUMA is a premier service laboratory in the US open to any researcher investigating solution behavior of macromolecules, polymers, nanoparticles and assemblies. Dr. Demeler continued his development of AUC analysis software, securing multi-million $ funding through NIH and NSF grants to create the most advanced AUC analysis software available today, called UltraScan. Dr. Demeler was the first to employ high-performance computing to maximize resolution, information content and increase throughput for AUC analysis. He has authored over 130 manuscripts and book chapters, about half of them on methods development for AUC. Dr. Demeler currently co-develops the XSEDE Science Gateway, a $5 Mio. NSF grant with Indiana University and San Diego Supercomputing Center to advance access to high-performance computing for AUC and a a broad range of other science domains. He holds an annual AUC workshop in San Antonio where he teaches the foundation of hydrodynamics and mentors future AUC experts.

    Abstract

    DATE: June 16, 2020

    TIME:  8:00am PT

    Multi-wavelength analysis of hydrodynamic data collected on the latest analytical ultracentrifugation instruments provides additional resolution and insights into hetero-interacting systems by adding spectral characterization to the traditional hydrodynamic separation of different molecules present in a mixture. 

    In this talk Dr. Demeler will provide an introduction into this new methodology, its computational challenges, and explain its benefits for the investigation of biopolymer interactions in the solution phase. The technique is used to exploit different chromophores present in different chemicals interacting with each other in solution, such as proteins and nucleic acids, proteins with variable ratios of amide backbone absorbance compared to tryptophan/tyrosine absorbance, proteins linked to fluorescent proteins, or proteins containing unique chemical groups like metal-bound porphyrins, or fluorescent tags. Dr. Demeler will also illustrate this advance by presenting several examples from his laboratory, covering situations commonly encountered in the characterization of biomolecular interactions.

     

    Learning Objectives:

    • Understanding the study of heterointeracting biopolymers

    • Understanding the value of multi-wavelength analysis

    • Performing multi-wavelength analysis with the Optima AUC

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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