APR 13, 2017 07:30 AM PDT

The Essential Role of the Molecular Identification of Microorganisms in the Management of Chronic Infections

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Medical Director, Southwest Regional Wound Care Center
    Biography
      Dr. Randall Wolcott is the founder of the Southwest Regional Wound Care Center in Lubbock, TX. The Wound Care Center specializes in biofilm-based wound care. The Wound Care Center offers state of the art technology with molecular diagnostics and state of the art alternative medicine including customized topical wound gels. He is passionate about healing wounds.

    Abstract:

    Microorganisms growing in biofilm phenotype are the cause of chronic infections.  Several universal properties of biofilm communities are they are overwhelmingly polymicrobial, difficult to culture (viable but not culturable), and the individual constituents are very tolerant of antimicrobial agents, especially antibiotics.  Just like in medical imaging utilizes multiple technological platforms such as ultrasound, magnetic resonance or x-ray, microbial identification requires multiple technologies and methods.  However, when dealing with biofilm phenotype microorganisms, molecular methods are superior.  Molecular methods have the ability to identify microorganisms with very high sensitivity, specificity and also can be quantitative.  Utilizing a quantitative PCR methodology, specific targets can be identified as present/not present but also an absolute quantitative determination for each target present can be made.  This allows for the determination of “bacterial load”.  Sequencing platforms can reveal crucial relative quantification of the different microorganisms present, allowing for the determination of dominant microbes.  Timely and accurate diagnostic information of microorganisms, which are the primary cause of the chronic infection, are essential for improving outcomes.  PCR information can be returned rapidly, allowing the clinician to rule out antibiotic resistant microbes which allows for the selection of appropriate empiric antibiotics.  Sequencing data, which returns several days later, allows for great sensitivity and specificity in the identification of microorganisms while also providing the information on what is dominant within the community.  Antibiotic therapy can then be tailored to the chronic infection.  Molecular diagnosis also provides a way to assess the efficacy of treatment.  Chronic infections are more prevalent and costly than heart disease, cancer, diabetes or any other disease in our healthcare system.  Molecular methods for identifying microorganisms provide a powerful diagnostic tool for the management of chronic infections. 


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    SEP 12, 2017 08:00 AM PDT
    SEP 12, 2017 08:00 AM PDT
    DATE: September 12, 2017TIME: 8:00am PT, 11:00am ETEvery year, millions of dollars are wasted on poorly characterized and performing antibodies.  Key researchers in the antibody co...
    NOV 15, 2018 10:00 AM PST
    C.E. CREDITS
    NOV 15, 2018 10:00 AM PST
    DATE:  November 15, 2018TIME:  10:00am PT, 1:00pm ET Multiplex fluorescence immunohistochemistry offers a window into the biology of human disease, enabling the ana...
    Loading Comments...