JUL 23, 2019 10:00 AM PDT

Molecularly Guided High Plex Spatial Profiling in the Tumor Microenvironment

Speakers
  • Principal Product Application Scientist, NanoString
    Biography
      DOUG HINERFELD, PH.D. is the Principal Product Application Scientist for NanoString 3D Biology™ Technology. In this role at NanoString, he is responsible for the development of novel applications for the 3D Biology portfolio. He received a B.A. in Psychology from The University of Colorado, Boulder and a Ph.D. in Genetics and Molecular Biology from Emory University.
    • Senior Scientist at NanoString Technologies, Inc.
      Biography
        Dr. Chris Merritt is a molecular biologist with expertise in developmental biology and cancer research. Currently, he is the lead R&D scientist for developing new multiplexing technologies and lyse-n-go optimized protocols for gene expression analysis, along with many other novel technologies at NanoString. He received his Ph.D. in Molecular Biology and Genetics from the Johns Hopkins School of Medicine where he studied gene expression in the C. elegans germline.
      • Application Scientist - Advanced Cell Diagnostics
        Biography
          Anushka Dikshit, Ph.D. is an Application Scientist with Advanced Cell Diagnostics with background in cancer research and immuno-oncology. She is focused on expanding the applications of RNAscope technology for oncology research. Prior to working for ACD, Anushka was involved in identifying novel therapeutic targets for melanoma as a postdoctoral fellow at Duke University. She obtained her doctoral degree from Southern Illinois University, School of Medicine in Molecular Physiology.

        Abstract

        DATE: July 23, 2019

        TIME: 10:00am PDT

        The tumor microenvironment is a complex network of different cell types and states that imparts distinct properties to the tumor and plays a significant role in determining the efficacy of cancer therapies. Immunotherapies in particular have demonstrated considerable therapeutic efficacy and durable response for several tumor types, however the majority of patients are resistant or relapse after initial response. More precise spatial characterization of the tumor and tumor microenvironment with high multiplexing can potentially provide critical insight into new immunotherapeutic strategies and identify new predictive biomarkers for stratifying patients most likely to benefit from immunotherapy.

        Using the RNAscope® in situ hybridization (ISH) technology from Advanced Cell Diagnostics, Inc. to molecularly guide Nanostring’s GeoMx™ Digital Spatial Profiler (DSP), it is now possible to characterize the tumor and the tumor microenvironment using 1,500+ RNA probes. The single molecule and single cell capabilities of the RNAscope ISH assay allows researchers to select discreate regions of interest for high plex spatial analyses of RNA with the highly multiplexed spatial profiling capabilities of GeoMx DSP.

        In this webinar we will demonstrate how RNAscope ISH assays can be combined with GeoMx DSP to assess expression of RNA from a specific regions of interest and perform follow up confirmation of target gene expression at the single-cell level, all with spatial context.

        Key learning objectives:

        • Introduction to GeoMx DSP and RNAscope ISH technologies
        • Learn detailed workflow for molecularly guided high plex spatial analysis
        • Learn Compatibility and correlation between GeoMx DSP and RNAscope ISH
        • Confirmation of GeoMx DSP results with RNAscope ISH at the single-cell level

         

        Webinars will be available for unlimited on-demand viewing after live event.

         

        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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