MAY 13, 2015 01:30 PM PDT

More from Less- High Quality Data from Low Quality Samples

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  • Product Marketing Manager, Target Enrichment, New England Biolabs
      Andrew Barry is the Product Marketing Manager for Target Enrichment at New England Biolabs. He has a B.S. in Biology from Stonehill College, and Master of Science in Biomedical Engineering from Tufts University. Prior to joining NEB Andrew spent the first 10 years of his career at the Broad Institute of MIT and Harvard where he developed high-throughput processes for genomic applications. Following his time at the Broad, he joined Caliper Life Sciences (later acquired by PerkinElmer) where he held various positions in product management, strategic marketing, and R&D. Andrew joined New England Biolabs in 2016.


    The continued growth of DNA sequencing as a fundamental data output has driven the need for the ability to generate high quality data from an increasing breadth of diminishing primary sample inputs. As researchers and clinicians continue to develop associations between nucleic acid variants and disease and treatment response mechanisms, it has become increasingly important to maximize the data yield from available sample materials. The Broad Institute Genomics Platform has developed several strategies to mitigate the impact of low quality or low input sample materials on the data integrity for sample and cohort studies. These include improved protocols for nucleic acid extraction and sample preparation, implementation of quality control assays, and improved analytical tools for the identification and filtering of data artifacts. Here, we will discuss the types of quality issues that we observe, and the corresponding strategies that have been developed to overcome these.

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