MAY 13, 2015 1:30 PM PDT

More from Less- High Quality Data from Low Quality Samples

Speaker
  • Product Marketing Manager, Target Enrichment, New England Biolabs
    Biography
      Andrew Barry is the Product Marketing Manager for Target Enrichment at New England Biolabs. He has a B.S. in Biology from Stonehill College, and Master of Science in Biomedical Engineering from Tufts University. Prior to joining NEB Andrew spent the first 10 years of his career at the Broad Institute of MIT and Harvard where he developed high-throughput processes for genomic applications. Following his time at the Broad, he joined Caliper Life Sciences (later acquired by PerkinElmer) where he held various positions in product management, strategic marketing, and R&D. Andrew joined New England Biolabs in 2016.

    Abstract

    The continued growth of DNA sequencing as a fundamental data output has driven the need for the ability to generate high quality data from an increasing breadth of diminishing primary sample inputs. As researchers and clinicians continue to develop associations between nucleic acid variants and disease and treatment response mechanisms, it has become increasingly important to maximize the data yield from available sample materials. The Broad Institute Genomics Platform has developed several strategies to mitigate the impact of low quality or low input sample materials on the data integrity for sample and cohort studies. These include improved protocols for nucleic acid extraction and sample preparation, implementation of quality control assays, and improved analytical tools for the identification and filtering of data artifacts. Here, we will discuss the types of quality issues that we observe, and the corresponding strategies that have been developed to overcome these.


    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    JUN 17, 2020 1:30 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 17, 2020 1:30 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Understanding the complex interplay between a pathogen and the host response is important to developing effective vaccines and therapeutics. The nCounter® Analysis System and GeoMx®...
    FEB 19, 2020 11:00 AM PST
    C.E. CREDITS
    FEB 19, 2020 11:00 AM PST
    DATE: February 19, 2020TIME: 11:00am PST, 2:00pm EST...
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    Loading Comments...
    Show Resources