OCT 24, 2019 6:00 AM PDT

Multidimension Chromatography; a Disruptive Technology - A Netflix Effect in the World of Chromatography!

Speaker

Abstract

Chromatography is the first choice in separation technique for a wide selection of applications, across many fields, ranging from simple to highly complex extracts.  The main rationale for its use is the resolution power, or peak capacity, and ultimately the separation recording of an extract into its distinct components. Thus, increasing the performance of chromatography is inevitably linked to improvements in peak capacity. With this goal in mind, innovative researchers lead the introduction of sub 2 um particles (UPLC), core-shell particles, and monolithic stationary phases, and produced attainable performance of peak capacity up to a thousand for 1D LC separation.  However, from Giddings work, the actual peak capacity of any given single dimensional separation is still far below the theoretical calculation.

With new technologies, newer solutions emerge for the most difficult of applications.  The concept of hyphenated systems or multi-dimension chromatography was designed, at first, for solving complex analyses. The peak capacity, or chromatographic separation power, can be increased by combining several separation dimensions (in most cases, two) each using optimized conditions for maximum resolution.  The main challenge is the transfer of closely resolved analytes from the primary resolving dimension (PRD) to the secondary resolving dimension (SRD).  Nowadays, multi-dimensional-chromatography is gaining acceptance for both targeted and untargeted analysis of complex mixtures.  However, at its beginnings, the technique was perceived as highly complex in terms of the necessary hardware and difficulty to gain in-depth insight for practical usage.  

In recent years, advances in software control enabled hyphenated instrumentation platforms with enhanced performance and relative ease of use. Additionally, multi-fluidic circuits have been shown to decrease the cost and time of sample preparation, improve chromatographic peak shape, and enhance sensitivity.  In this presentation, several forensic and environmental applications will be discussed to fully demonstrate the capabilities of modern 2D LC/MS/MS workflows.  

Learning objectives:

1. Novel extraction techniques for wide range of applications
2. Exploring the world Multidimension liquid chromatography and the myth of a technique difficult to master.  

 


Show Resources
You May Also Like
JUN 28, 2022 6:45 AM PDT
JUN 28, 2022 6:45 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
DEC 09, 2021 6:00 AM PST
C.E. CREDITS
DEC 09, 2021 6:00 AM PST
Date: December 9, 2021 Time: 6:00am (PDT), 9:00 am (EDT) As the number of biotherapeutic candidates in development is increasing, the requirements of analytical tools to understand the molec...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
MAR 16, 2022 9:00 AM PDT
C.E. CREDITS
MAR 16, 2022 9:00 AM PDT
Date: March 16, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEDT) With a renewed and growing interest in therapeutic oligonucleotides (oligos) across the pharmaceutical industry, the req...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
OCT 24, 2019 6:00 AM PDT

Multidimension Chromatography; a Disruptive Technology - A Netflix Effect in the World of Chromatography!



Show Resources
Loading Comments...
Show Resources