FEB 01, 2017 8:00 AM PST

WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

Sponsored by: SCIEX
Speaker
  • Associate Director Analytical Testing Support at Halozyme Therapeutics Inc.
    Biography
      Laura Salmeron has more than 25 years of experience working in the Clinical and Biotechnology field supporting protein therapeutics. Received her B.S. in Biochemistry and Microbiology and Medical Technologist from the University Autonoma of Guadalajara, Mexico. Worked in Rx Medical Labs in Monrovia CA for 5 years, thereafter owned a Clinical Laboratory in Hospital San Jose in Tijuana BC, Mexico. In 1995 joined Biogen Idec in the Analytical Sciences group, then moved to the Analytical Development as the Lead for the San Diego site, where she worked for 15 years. In 2011 Laura joined the Drug Product group at Amgen Thousand Oaks, CA as the Process Lead for the Epogen program then transferred to the Stability group as a Lead for all BITE molecules. In 2015 Laura joined Halozyme Therapeutics in San Diego CA, working with Halozyme Enhance platform partners and leading the Potency group and as a key member of the Analytical Development team working with FIH, Clinical and Commercial products supporting method transfers at CRO's and CMO's and regulatory submissions.

    Abstract

    DATE: February 1, 2017
    TIME: 8:00AM PT, 11:00AM ET

    In this presentation the complementarity of various liquid phase bioseparation methods including capillary electrophoresis and liquid chromatography are discussed. N‐glycosylation analysis of a development‐phase glycoprotein having multiple glycosylation sites using these orthogonal methods demonstrated that complementarity is the key to solving the glycosylation puzzle. Most structures were simply identified by public database search either by Expasy or GUcal, based on their molecular weight or measured glucose unit values. Glycan structures not included in the GuCal database were deciphered by exoglycosidase‐based glycan sequencing, to sequentially remove the sugar residues from the nonreductive end of the glycan structures.

    Learning Objective 1: Introduce the New, Fast Glycan Labeling & Analysis Kit from SCIEX

    Learning Objective 2: Discover 60 Minute Sample Prep for Up To 96 Glycan Samples

    Learning Objective 3: Learn How to Separate and Identify N-Glycans in Minutes

    Learning Objective 4: Compare Liquid Phase Separation Methods for N-Glycan Analysis


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