FEB 01, 2017 8:00 AM PST

WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

Sponsored by: SCIEX
Speaker
  • Associate Director Analytical Testing Support at Halozyme Therapeutics Inc.
    Biography
      Laura Salmeron has more than 25 years of experience working in the Clinical and Biotechnology field supporting protein therapeutics. Received her B.S. in Biochemistry and Microbiology and Medical Technologist from the University Autonoma of Guadalajara, Mexico. Worked in Rx Medical Labs in Monrovia CA for 5 years, thereafter owned a Clinical Laboratory in Hospital San Jose in Tijuana BC, Mexico. In 1995 joined Biogen Idec in the Analytical Sciences group, then moved to the Analytical Development as the Lead for the San Diego site, where she worked for 15 years. In 2011 Laura joined the Drug Product group at Amgen Thousand Oaks, CA as the Process Lead for the Epogen program then transferred to the Stability group as a Lead for all BITE molecules. In 2015 Laura joined Halozyme Therapeutics in San Diego CA, working with Halozyme Enhance platform partners and leading the Potency group and as a key member of the Analytical Development team working with FIH, Clinical and Commercial products supporting method transfers at CRO's and CMO's and regulatory submissions.

    Abstract

    DATE: February 1, 2017
    TIME: 8:00AM PT, 11:00AM ET

    In this presentation the complementarity of various liquid phase bioseparation methods including capillary electrophoresis and liquid chromatography are discussed. N‐glycosylation analysis of a development‐phase glycoprotein having multiple glycosylation sites using these orthogonal methods demonstrated that complementarity is the key to solving the glycosylation puzzle. Most structures were simply identified by public database search either by Expasy or GUcal, based on their molecular weight or measured glucose unit values. Glycan structures not included in the GuCal database were deciphered by exoglycosidase‐based glycan sequencing, to sequentially remove the sugar residues from the nonreductive end of the glycan structures.

    Learning Objective 1: Introduce the New, Fast Glycan Labeling & Analysis Kit from SCIEX

    Learning Objective 2: Discover 60 Minute Sample Prep for Up To 96 Glycan Samples

    Learning Objective 3: Learn How to Separate and Identify N-Glycans in Minutes

    Learning Objective 4: Compare Liquid Phase Separation Methods for N-Glycan Analysis


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    MAR 18, 2021 8:00 AM PDT
    C.E. CREDITS
    MAR 18, 2021 8:00 AM PDT
    DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
    FEB 24, 2021 10:00 AM PST
    C.E. CREDITS
    FEB 24, 2021 10:00 AM PST
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    APR 08, 2021 11:00 AM PDT
    C.E. CREDITS
    APR 08, 2021 11:00 AM PDT
    Date: April 8, 2021 Time: 11:00am (PDT), 3:00pm (EDT) Pneumonia is a deadly condition with clinical outcomes highly dependent on prompt and appropriate therapy. Diagnosis of pneumonia is cha...
    FEB 01, 2017 8:00 AM PST

    WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

    Sponsored by: SCIEX

    Specialty

    Biotechnology

    Biochemistry

    Molecular Biology

    Cancer Research

    Cell Biology

    Biomarkers

    Analytical Chemistry

    Clinical Diagnostics

    Molecular Diagnostics

    Immunology

    Cancer Therapeutics

    Laboratory Testing

    Clinical Chemistry

    Microbiology

    Antibodies

    Geography

    North America52%

    Europe27%

    Asia13%

    South America2%

    Africa2%

    Registration Source

    Website Visitors100%

    Job Title

    Scientist23%

    Executive15%

    Research Scientist14%

    Facility/Department Manager12%

    Student7%

    Educator/Faculty7%

    Medical Laboratory Technician4%

    Medical Doctor/Specialist4%

    Lab Management4%

    Post Doc4%

    Chemist3%

    Marketing/Sales2%

    Clinical Laboratory Scientist1%

    Biologist1%

    Organization

    Academic Institution8%

    Life Science Company5%

    Biotech Company5%

    Research Institute4%

    Hospital4%

    Pharmaceutical Company3%

    Clinical Laboratory3%

    Government2%

    Industrial Company2%

    Contract Research Organization (Cro)2%

    Consultant2%

    Non-Profit Organization1%

    Ambulatory Care1%

    Medical School1%

    Other4%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more