FEB 01, 2017 8:00 AM PST

WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

Sponsored by: SCIEX
Speaker

Abstract

DATE: February 1, 2017
TIME: 8:00AM PT, 11:00AM ET

In this presentation the complementarity of various liquid phase bioseparation methods including capillary electrophoresis and liquid chromatography are discussed. N‐glycosylation analysis of a development‐phase glycoprotein having multiple glycosylation sites using these orthogonal methods demonstrated that complementarity is the key to solving the glycosylation puzzle. Most structures were simply identified by public database search either by Expasy or GUcal, based on their molecular weight or measured glucose unit values. Glycan structures not included in the GuCal database were deciphered by exoglycosidase‐based glycan sequencing, to sequentially remove the sugar residues from the nonreductive end of the glycan structures.

Learning Objective 1: Introduce the New, Fast Glycan Labeling & Analysis Kit from SCIEX

Learning Objective 2: Discover 60 Minute Sample Prep for Up To 96 Glycan Samples

Learning Objective 3: Learn How to Separate and Identify N-Glycans in Minutes

Learning Objective 4: Compare Liquid Phase Separation Methods for N-Glycan Analysis


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
AUG 24, 2021 11:00 AM PDT
C.E. CREDITS
AUG 24, 2021 11:00 AM PDT
Date: August 24, 2021 Time: 11:00am (PDT), 1:00pm (EDT) Electron-based dissociation mechanisms have shown great promise for advanced characterization of biomolecules. However, routine adopti...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
FEB 01, 2017 8:00 AM PST

WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

Sponsored by: SCIEX


Show Resources
Loading Comments...
Show Resources