DATE: February 1, 2017
TIME: 8:00AM PT, 11:00AM ET
In this presentation the complementarity of various liquid phase bioseparation methods including capillary electrophoresis and liquid chromatography are discussed. N‐glycosylation analysis of a development‐phase glycoprotein having multiple glycosylation sites using these orthogonal methods demonstrated that complementarity is the key to solving the glycosylation puzzle. Most structures were simply identified by public database search either by Expasy or GUcal, based on their molecular weight or measured glucose unit values. Glycan structures not included in the GuCal database were deciphered by exoglycosidase‐based glycan sequencing, to sequentially remove the sugar residues from the nonreductive end of the glycan structures.
Learning Objective 1: Introduce the New, Fast Glycan Labeling & Analysis Kit from SCIEX
Learning Objective 2: Discover 60 Minute Sample Prep for Up To 96 Glycan Samples
Learning Objective 3: Learn How to Separate and Identify N-Glycans in Minutes
Learning Objective 4: Compare Liquid Phase Separation Methods for N-Glycan Analysis