FEB 01, 2017 08:00 AM PST

WEBINAR: N‐Linked Glycosylation Profiling: Rapid Separation Techniques for the Analysis of a Development‐Phase Therapeutic Glycoprotein

SPONSORED BY: SCIEX
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Associate Director Analytical Testing Support at Halozyme Therapeutics Inc.
    Biography
      Laura Salmeron has more than 25 years of experience working in the Clinical and Biotechnology field supporting protein therapeutics. Received her B.S. in Biochemistry and Microbiology and Medical Technologist from the University Autonoma of Guadalajara, Mexico. Worked in Rx Medical Labs in Monrovia CA for 5 years, thereafter owned a Clinical Laboratory in Hospital San Jose in Tijuana BC, Mexico. In 1995 joined Biogen Idec in the Analytical Sciences group, then moved to the Analytical Development as the Lead for the San Diego site, where she worked for 15 years. In 2011 Laura joined the Drug Product group at Amgen Thousand Oaks, CA as the Process Lead for the Epogen program then transferred to the Stability group as a Lead for all BITE molecules. In 2015 Laura joined Halozyme Therapeutics in San Diego CA, working with Halozyme Enhance platform partners and leading the Potency group and as a key member of the Analytical Development team working with FIH, Clinical and Commercial products supporting method transfers at CRO's and CMO's and regulatory submissions.

    Abstract:

    DATE: February 1, 2017
    TIME: 8:00AM PT, 11:00AM ET

    In this presentation the complementarity of various liquid phase bioseparation methods including capillary electrophoresis and liquid chromatography are discussed. N‐glycosylation analysis of a development‐phase glycoprotein having multiple glycosylation sites using these orthogonal methods demonstrated that complementarity is the key to solving the glycosylation puzzle. Most structures were simply identified by public database search either by Expasy or GUcal, based on their molecular weight or measured glucose unit values. Glycan structures not included in the GuCal database were deciphered by exoglycosidase‐based glycan sequencing, to sequentially remove the sugar residues from the nonreductive end of the glycan structures.

    Learning Objective 1: Introduce the New, Fast Glycan Labeling & Analysis Kit from SCIEX

    Learning Objective 2: Discover 60 Minute Sample Prep for Up To 96 Glycan Samples

    Learning Objective 3: Learn How to Separate and Identify N-Glycans in Minutes

    Learning Objective 4: Compare Liquid Phase Separation Methods for N-Glycan Analysis


    Show Resources
    You May Also Like
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JUN 26, 2019 09:00 AM PDT
    C.E. CREDITS
    JUN 26, 2019 09:00 AM PDT
    DATE: June 26, 2019TIME: 9:00am PDT, 12:00pm EDT An excessive number of software solutions are available to help manage your clinical, biobank, or biorepository sample inform...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    Loading Comments...
    Show Resources