DEC 09, 2020 8:00 AM PST
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Nanoscopy Meets Lifetime - Introducing the new STELLARIS 8 τ-STED

Sponsored by: Leica Microsystems
Speaker
  • Product Manager-Advanced Confocal Imaging
    Biography
      Julia studied Chemistry at the University of Buenos Aires, where she worked on Photochemistry and Analytical Chemistry. She focused on the characterization of luminescence of lanthanide complexes, and on the development of boron quantification methods for boron neutron capture therapy (BNCT) of cancer. After graduating, she moved to Göttingen to carry out her doctoral research at the Max Planck Institute for Biological Chemistry. She developed in vitro and in situ fluorescence labeling strategies to elucidate the oligomerization and aggregation mechanisms of the Parkinson's disease-associated protein alpha-synuclein. In 2012, she joined EMBL as Humboldt postdoctoral fellow, and applied advanced confocal microscopy and nanoscopy imaging to study chromatin compaction at the interphase-to-mitosis transition. She joined Leica Microsystems in 2017 as Product Manager for advanced confocal imaging.

    Abstract
    Date:  December 9, 2020
    Time: 8:00am (PST),  11:00am (EST)
     
    This talk introduces the Leica STELLARIS 8 τ-STED system where STED Nanoscopy meets Leica’s Fast Lifetime Contrast (FALCON) imaging to enable a new level of super resolution performance. Since the advent of super-resolution fluorescence microscopies, the characterization of macromolecular complexes and cellular structures at the sub-diffraction level has become a reality and STED Nanoscopy has become an accepted confocal-based technology to deliver resolutions in the tens of nm. τ-STED combines the optical signals from STED imaging with the photophysical information from the fluorescence lifetime at unprecedented speed and sensitivity. τ-STED uses phasor analysis in a novel way to enable increased STED resolution and elimination of uncorrelated background noise even with reduced excitation and depletion powers. This talk will also present some interesting application examples, including 2D and 3D STED in live and in fixed specimens, as well as for multicolor applications.
     
    Learning Objectives
    • Learn how Leica’s NEW τ-STED uses phasor analysis in a novel way to enable increased STED resolution and elimination of uncorrelated background noise
    • Learn how Leica’s NEW τ-STED allows for lower excitation and STED light dose to protect your sample, extend time-lapse experiments, and enable large volume imaging
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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