MAY 28, 2020 8:00 AM PDT

Novel Roles of Actin Cytoskeleton on Mitochondrial Dynamics and Homeostasis

Sponsored by: Andor
C.E. Credits: Florida CE P.A.C.E. CE
Speakers

Abstract
DATE:  May 28, 2020
TIME:   8AM PT, 11AM ET, 4PM GMT, 5PM CET
 
Mitochondria have traditionally been viewed as energy-generating organelles, through oxidation of metabolic substrates and creation of a proton gradient across the inner mitochondrial membrane (IMM), that is utilized to generate ATP. However, it is increasingly clear that mitochondria communicate frequently with the rest of the cell and are therefore important signaling organelles. Mitochondria are extremely dynamic organelles; that fuse and divide continuously. Failures in mitochondrial dynamics cause severe disorders, and therefore the study of such mechanisms has significant importance.
 
A growing number of studies suggests that actin polymerization participates in mitochondrial communication and dynamics in mammalian cells. We find that there are at least two distinct modes by which actin and mitochondria interact.
  • In one mode (Chakrabarti et al (2019) J. Cell Biol. 217(1): 251-268), elevation of cytosolic calcium activates the endoplasmic reticulum (ER)-bound formin INF2, and the resulting actin polymerization stimulates two distinct events that promote mitochondrial division: increased transfer of calcium from ER to mitochondria, (resulting in IMM constriction); and Drp1 recruitment to the outer mitochondrial membrane (OMM), resulting in OMM constriction.
  • In the other mode (Fung et al (2019) J. Cell Sci.), mitochondrial depolarization causes rapid accumulation of an extensive ‘shell’ of actin filaments around the depolarized mitochondria, dependent on Arp2/3 complex but independent of INF2. This actin shell selectively and subtly unwraps the ER from mitochondria and delays the initial phases of the Pink/Parkin dependent mitophagy pathway.
To achieve the mechanistic information on actin’s role in mitochondrial homeostasis, we have used ANDOR Dragonfly ultra-fast imaging system. Dragonfly provides the perfect solution for imaging live or fixed mitochondria.
 
In this webinar, we will present our recent results about on the role of actin cytoskeletal on mitochondrial dynamics. Mitochondria imaging analysis tools will also be discussed.
 
Learning Objectives:
  • Discover the role of ER in regulation mitochondrial dynamics
  • Uncover the role of actin cytoskeleton in stimulating dynamics of both mitochondrial membranes
  • Identify the difficulties in imaging live mitochondria
  • Understand strategies for image live mitochondria
  • Acquire knowledge on biological imaging analysis.
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
 
 

 

 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
AUG 24, 2021 11:00 AM PDT
C.E. CREDITS
AUG 24, 2021 11:00 AM PDT
Date: August 24, 2021 Time: 11:00am (PDT), 1:00pm (EDT) Electron-based dissociation mechanisms have shown great promise for advanced characterization of biomolecules. However, routine adopti...
Loading Comments...
Show Resources