MENU
APR 02, 2020 2:30 PM PDT

Opportunities for using PacBio Long-read Sequencing for COVID-19 Research

Sponsored by: PacBio
Speaker

Abstract

Scientists worldwide are actively working to tame the COVID-19 pandemic by developing therapies for patients with severe disease and a vaccine to stem transmission. Accomplishing these two goals will require high resolution genetic information on both the SARS-Cov-2 virus and the host response to infection, beyond what suffices for diagnostic testing.

PacBio highly accurate long-read sequencing data is one tool scientists can use in their COVID-19 research. PacBio sequencing is unique in that it produces HiFi reads that are both long (up to 15 kb) and have very high base-level accuracy (>99.9%). These qualities are advantageous for applications that require phasing of SNVs across DNA amplicons and for resolving individual variants within complex populations of highly similar sequences. For example, in order to develop a broadly efficacious therapeutic or vaccine, scientists will need to understand how SARS-CoV-2 evolves within a host, over time in a community, and across geographic regions. An examination of currently public SARS-CoV-2 genomes shows how challenging getting high quality data can be with other sequencing approaches. Similarly, when mining the immune repertoire of recovered patients for therapeutics, researchers will need accurate and complete information on B cell receptors that can carry SNVs and indels along the entire length of the variable region. Furthermore, PacBio sequencing can directly detect and phase the germline IGH locus from which B cell receptors are derived, allowing researchers to connect phenotype to genotype without inference. In this presentation, we will discuss what PacBio sequencing can offer COVID-19 researchers as they strive to understand the biology underlying this global pandemic.

Learning Objectives:

1. Understanding how highly accurate long reads can be used to obtain high quality SARS-CoV-2 genomes with no gaps, and how this connects to virus research goals

2. Understand how highly accurate long reads can be used to understand the host immune response to SARS-CoV-2 infection.


Show Resources
You May Also Like
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAY 04, 2022 10:30 AM PDT
C.E. CREDITS
MAY 04, 2022 10:30 AM PDT
The coronavirus SARS-CoV-2 has led to a worldwide pandemic. Beside respiratory symptoms, infected people may develop neurologic dysfunctions such as loss of smell and taste, also seizures, a...
MAY 19, 2022 8:00 AM PDT
MAY 19, 2022 8:00 AM PDT
Date: May 19, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classific...
Loading Comments...
Show Resources