OPT-ical Tomography for Developmental Biologists

Speaker
  • Head of Advanced Imaging at Instituto Gulbenkian de Ciencia - Oeiras, Portugal, Professor of Bioimaging, Faculty of Science, University of Lisbon

Abstract

Almost unidimensional in their primordial existence as single cells, multicellular organisms quickly develop into complex 3D architectures and machineries composed of multiple cells and tissues. For more than a century, classical microscopy has given us important insights into the mechanics and mechanisms that bring about complex biological forms during their development, but it was only recently that we have been able actually witness those processes it their full complexity and wonder. This was due mainly to the emergence of 3D optical microscopy techniques, such as confocal or two-photon, and more recently of mesoscopic imaging such as light-sheet and optical tomography. I will quickly review some of the principles behind optical tomography/slicing and its use in cell and developmental biology and focus on optical projection tomography (OPT) as a tool for embryologists used to characterize vertebrate embryonic and fetal anatomy with little efforts and great fidelity.


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAY 19, 2022 8:00 AM PDT
MAY 19, 2022 8:00 AM PDT
Date: May 19, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classific...
JUN 29, 2022 7:00 AM PDT
JUN 29, 2022 7:00 AM PDT
Date: June 29, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Imaging live cells is key to reveal their inner workings, dynamics, and function. Current technological advances in confo...
AUG 10, 2022 10:00 AM PDT
AUG 10, 2022 10:00 AM PDT
Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
Loading Comments...
Show Resources
Attendees