Optimize your PCR

Welcome to this exciting webinar: Optimize your PCR, where we will delve into the intricacies of this powerful molecular biology technique. Our speaker, Gabriel Almeida Alves, BSN, MS, PhD, will take us on a journey through the world of PCR, exploring its various components, steps, and troubleshooting methods.

Join us as we delve into the world of PCR and learn about:

• DNA Template and Purification: Learn about the importance of a pure and high-quality DNA sample for efficient PCR amplification.

• DNA Polymerases and Buffers: Explore the different types of DNA polymerases and their respective buffers that are commonly used in PCR reactions.

• Oligo Design - The Ideal Primers: Understand the critical role of primers in the PCR reaction and learn how to design the ideal primer set.

• Choosing the Right Plastics: Find out the significance of plastics in PCR and learn how to choose the appropriate plasticware for your experiment.

• Thermal Cyclers: Discover the different types of thermal cyclers and their respective advantages and limitations.

• Post-PCR Workflow - Electrophoresis: Learn about the crucial post-PCR analysis step of electrophoresis and how it helps us to visualize and analyze the amplified DNA product.

This webinar will provide you with an in-depth understanding of PCR, its various components, and their importance. You will also learn about common pitfalls of PCR and how to avoid or troubleshoot them. We hope that this webinar will be a valuable resource for both new and experienced researchers alike, and help you take your PCR experiments to the next level!

Learning Objectives:

1. DNA Template and Purification: Discuss the importance of a pure and high-quality DNA sample for efficient PCR amplification.

2. DNA Polymerases and Buffers: Explain the different types of DNA polymerases and their respective buffers that are commonly used in PCR reactions.

3. Thermal Cyclers: Discover the different types of thermal cyclers and their respective advantages and limitations.