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APR 21, 2020 10:30 AM PDT

Optimizing human karyomapping to phase single lgene defects with improved DNA amplification

Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Senior Global Product Manager, RNA Profiling , QIAGEN
    Biography
      Samuel Rulli is a Senior Global Product Manager for the QIAseq RNA Profiling Technologies at QIAGEN. Dr. Rulli received his Ph.D. in 2002 from Tulane University studying the gastric proton pump and did post-doctoral research at Johns Hopkins University and the National Cancer Institute in Frederick, MD. He joined SABiosciences - now a part of QIAGEN - in 2007 as an R&D Scientist in qPCR applications and moved to a Global Product Manager role in 2014. Trained as a molecular biologist, Dr. Rulli has worked on different assay detection technologies for gene expression and nucleic acid analysis.

    Abstract

    Karyomapping, a technique for preimplantation genetic diagnosis (PGD) of single gene disorders, involves using very few or single cells, and comes with challenges due to limited amounts of sample DNA. To overcome these issues, whole genome amplification (WGA) can be used to provide high quality amplified DNA for karyomapping applications - but reliable WGA performance is necessary for accurate results.

    In this webinar, we'll discuss the REPLI-g Advanced DNA Single Cell kit for whole genome amplification using MDA (multiple Displacement Amplification) technology and its use in karyomapping applications. Get comprehensive overview of how MDS works, the features and workflow of the new kit, user feedback and several case study examples.

    Learning Objectives:

    1. Overview of Multiple Displacement Amplification (MDA) technology

    2. Discussion of performance data and REPLI-g workflow

    3. Review of two case studies


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