JAN 22, 2019 7:00 AM PST

Overcoming the challenges of accuracy and detection in molecular assays

Speaker
  • Senior Business Development Manager, Molecular Diagnostics, Licensing and Commercial Supply-UK, Ireland and Nordics
    Biography
      Peter's current role is in supporting customers who purchase Thermo Fisher Scientific products for commercial use, with a particular focus in supporting the use of molecular biology and sample preparation products for inclusion into diagnostic kits. He has over 15 years of experience in commercial sales and is responsible for Ion Torrent™ sequencing, capillary electrophoresis, real-time PCR, and molecular biology products. Prior to joining Thermo Fisher Scientific, Peter led a research group in molecular toxicology at AstraZeneca after receiving his doctorate in plant biology from the University of Edinburgh.

    Abstract
    DATE:    January 22, 2019
    TIME:    07:00am PST, 10:00am EST
     
    Molecular diagnostic tests are currently used for infectious disease testing due to the superior sensitivity and specificity that can be obtained. Due to the success that has been obtained with this application, molecular diagnostic testing is expected to grow into other areas such as genetic testing, prenatal testing, oncology, and pharmacogenomics. Growth in these areas will undoubtedly come with additional challenges due to the variety of the samples required, the presence of inhibitors, and low sample amounts, all of which contribute to potential false negative results due to poor amplification reactions.
     
    In this presentation, we will demonstrate how a change in assay components can overcome these challenges. Specifically, we will address the issues that the Nucleic Acid Amplification Test (NAAT) (a commonly used diagnostic tool used for its fast turnaround and less invasive sample collection) has faced and how a change in enzyme selection impacted the overall quality and accuracy of the molecular diagnostic assay.
     
    Learning Objectives:
    • Understand key challenges in nucleic acid amplification tests
    • Identify enzyme characteristics important for increased sensitivity, specificity, and speed
    • Understand how better PCR enzymes and reverse transcriptases can impact the accuracy and detection of molecular test assays
     

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